抑制半乳糖凝集素3促进椎间盘软骨终板细胞凋亡诱导椎间盘退变  

Inhibition of galectin-3 promotes apoptosis of cartilage endplate cells and induces intervertebral disc degeneration

作　　者：刘岩路[1] 胡炜[1] 艾克拜尔[1] 伊力亚 黄异飞[1] Liu Yanlu;Hu Wei;Aikebaier;Yiliya;Huang Yifei(Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University,Urumqi 830000,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆医科大学附属中医医院,新疆维吾尔自治区乌鲁木齐市830000

出　　处：《中国组织工程研究》2021年第35期5599-5603,共5页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区自然科学基金(2019D01C164),项目负责人:胡炜。

摘　　要：背景:椎间盘软骨终板的退变破坏了椎间盘完整性,影响了营养和代谢物交换及细胞外基质代谢平衡,是导致椎间盘退变的主要因素。半乳糖凝集素3(Galectin-3)是半乳糖凝集素家族的一员,参与调控细胞增殖、凋亡、细胞黏附等多种生理和病理过程,但是Galectin-3在椎间盘软骨终板中的调控作用尚不明确。目的:通过抑制剂GB1107干预Galectin-3,探讨其对大鼠软骨终板细胞增殖、凋亡、细胞周期的影响。方法:将大鼠软骨终板细胞分为正常对照组和Galectin-3 GB1107抑制剂组,其中GB1107抑制剂组分为6个浓度组,即1,2.5,5,10,25和50μmol/L组。MTT法检测3个时间点(12,24,48 h)各组椎间盘软骨终板细胞的增殖情况;采用最佳的抑制剂浓度25μmol/L进行后续流式测定,检测大鼠软骨终板细胞凋亡和细胞周期的差异变化。结果与结论:①Galectin-3抑制剂GB1107处理的大鼠软骨终板细胞的细胞增殖活性是浓度和作用时间依赖性的,在5-50μmol/L浓度范围内,3个时间段的细胞增殖活性较正常对照组明显降低(P<0.05);在25μmol/L浓度下,软骨终板细胞凋亡率显著高于正常对照组(P<0.05);②在抑制剂GB1107作用下,软骨终板细胞在G1期的数量显著高于正常对照组(P<0.05),在G2期和S期,抑制剂GB1107组的细胞数量显著低于正常对照组(P<0.05);③提示抑制Galectin-3可能会通过抑制软骨终板细胞DNA的复制,促进软骨终板细胞凋亡,导致椎间盘软骨终板的破坏,从而诱导椎间盘退变的发生和发展。BACKGROUND:Cartilage endplate degeneration of the intervertebral disc destroys the integrity of the intervertebral disc and impacts the exchange of nutrition and metabolites and the metabolic balance of extracellular matrix,which is the main factor leading to intervertebral disc degeneration.Galectin-3,a member of galectin family,is expressed in various tissues and participates in the regulation of cell proliferation,apoptosis,cell adhesion and other physiological and pathological processes.However,the regulatory role of Galectin-3 in the cartilage endplate of the intervertebral disc is still unclear.OBJECTIVE:To investigate the effects of Galectin-3 on the proliferation,apoptosis and cell cycle of rat cartilage endplate cells through the intervention of GB1107,a Galectin-3 inhibitor.METHODS:In this study,the rat cartilage endplate cells were divided into normal control group and Galectin-3 gb1107 inhibitor groups(1,2.5,5,10,25,and 50μmol/L).MTT was used to detect the proliferation of cartilage endplate cells in each group at three time points(12,24,48 hours).The optimal concentration of 25μmol/L was used for follow-up flow cytometry to detect the difference of cell apoptosis and cell cycle.RESULTS AND CONCLUSION:The proliferation activity of chondrocytes treated with GB1107 was concentration-and time-dependent.In the concentration range of 5-50μmol/L,the proliferation activity of chondrocytes was significantly lower than that of the normal control group at all three observational time points(P<0.05).At the concentration of 25μmol/L,the apoptosis rate of cartilage endplate cells was significantly higher than that of the normal control group(P<0.05).Under the effect of GB1107,the number of cartilage endplate cells at G1 stage was significantly lower than that in the normal control group(P<0.05),while the cell number at G2 and S stage was significantly lower than that in the normal control group(P<0.05).Thererfore,inhibition of Galectin-3 may inhibit the DNA replication of cartilage endplate cells to promote th

关 键 词：半乳糖凝集素3 GALECTIN-3 椎间盘退变 软骨终板 细胞增殖 细胞凋亡 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]