Wp-PKC对蚂蟥性腺发育影响的研究  

Effects of Wp-PKC on gonadal development of Whitmania pigra

作　　者：陈盼盼 郭巧生[1] 史红专[1] 张会宁 CHEN Pan-pan;GUO Qiao-sheng;SHI Hong-zhuan;ZHANG Hui-ning(Institute of Chinese Medicinal Materials,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学中药材研究所,江苏南京210095

出　　处：《中国中药杂志》2021年第6期1374-1378,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81673538,81473306)。

摘　　要：蛋白激酶C(PKC)是一类广泛参与细胞增殖和发育的激酶,蚂蟥体内PKC(Wp-PKC)属于经典PKC。为探究Wp-PKC对蚂蟥生殖细胞发育的影响,在蚂蟥养殖水体中分别添加:17β-雌二醇(17β-E2)(100 ng·mL^(-1))、甲基睾酮(MT)(150μg·L^(-1))、150μg·L^(-1)(MT)+0.5 mg·L^(-1) PKC、0.5 mg·L^(-1)PKC抑制剂和无添加组(对照组),观察对蚂蟥生殖细胞的发育和Wp-PKC表达的影响。结果表明:雄性腺中的Wp-PKC始终高于雌性腺;MT促进蚂蟥雄性腺发育的同时Wp-PKC表达量显著高于对照组;17β-E2促进蚂蟥雌性腺发育的同时Wp-PKC表达量显著低于对照组;PKC抑制剂组精巢和卵巢生殖细胞的发育显著慢于其他组,同时Wp-PKC表达量显著低于对照组。综上,Wp-PKC对蚂蟥性腺发育尤其雄性腺发育起了促进作用。Protein kinase C(PKC) is a kind of kinase which is widely involved in cell proliferation and development. PKC(Wp-PKC) in Whitmania pigra body belongs to classic PKC. In order to investigate the effect of Wp-PKC on the development of Wh. pigra germ cells, 17β-estradiol(17β-E2)(100 ng·mL^(-1)) and methyltestosterone(MT)(150 μg·L^(-1)), 150 μg·L^(-1)(MT)+0.5 mg·L^(-1) PKC, 0.5 mg·L^(-1) PKC inhibitor were added to Wh. pigra culture water, and no addition group(control group) was added, and the effects on the development of Wh. pigra germ cells and the expression of Wp-PKC were observed. The results showed that: Wp-PKC in male gonads was always higher than that in female gonads;MT promoted the development of male gonads in Wh. pigra, while the expression of Wp-PKC was significantly higher than that in the control;17β-E2 promoted the development of female gonads in Wh. pigra and Wp-PKC expression significantly lower than that of the control;while the development of the female and male gonads in the PKC inhibitor group was inhibited, the expression of Wp-PKC was significantly lower than that of the control. In summary, Wp-PKC may promote the development of Wh. pigra, especially the development of male gonads.

关 键 词：蚂蟥 甲基睾酮 17Β-E2 蛋白激酶C 生殖细胞 

分 类 号：S865.49[农业科学—野生动物驯养]