骨痹汤对骨关节炎模型大鼠血清炎性细胞因子及PI3K/Akt/mTOR信号通路的影响  

Effects of Gubi Decoction on serum related inflammatory factors and PI3K/Akt/mTOR signaling pathway in osteoarthritis model rats

作　　者：钟李青 丁少波 胡润凯 韩伟超 何淑芬 谢保城 Zhong Liqing;Ding Shaobo;Hu Runkai;Han Weichao;He Shufen;Xie Baocheng(Department of Pharmacy,Dongguan People’s Hospital Affiliated to Southern Medical University,Dongguan 523059,China)

机构地区：[1]南方医科大学附属东莞市人民医院药学部,523059

出　　处：《国际中医中药杂志》2021年第4期357-362,共6页International Journal of Traditional Chinese Medicine

基　　金：广东省医学科研基金(B2020086);广东省中医药局"十三五"中医重点、特色专科建设项目(2019年)。

摘　　要：目的探讨骨痹汤对骨关节炎模型大鼠血清相关炎性细胞因子水平及PI3K/Akt/mTOR信号通路的影响。方法将70只大鼠按随机数字表法分为空白组、假手术组、模型组、硫酸氨基葡萄糖组及骨痹汤高、中、低剂量组。除空白组和假手术组外,其余各组采用改良Hulth法制备膝骨关节炎大鼠模型。于模型制备成功后第28天,骨痹汤高、中、低剂量组分别灌胃骨痹汤24、12、6 g/kg;硫酸氨基葡萄糖组灌胃3 g/L硫酸氨基葡萄糖片混悬液,1次/d。连续灌胃28 d。采用ELISA法检测血清TNF-α、IL-1β水平,采用Real-PCR检测软骨组织中PI3K、Akt、mTOR基因表达,采用Western blot法检测PI3K、Akt、p-PI3K、p-Akt、mTOR蛋白表达。结果与模型组比较,硫酸氨基葡萄糖组与骨痹汤中、高剂量组大鼠膝关节直径[(11.17±1.81)mm、(11.60±1.38)mm、(10.80±1.17)mm比(12.57±0.98)mm]降低(P<0.05);骨痹汤中、高剂量组血清TNF-α[(111.43±21.98)ng/L、(53.42±13.25)ng/L比(157.89±23.60)ng/L]、IL-1β[(67.50±18.44)ng/L、(48.22±9.63)ng/L比(96.11±14.85)ng/L]水平降低(P<0.05),软骨组织PI3K[(1.87±0.17)、(1.24±0.49)比(2.19±0.47)]、Akt[(1.50±0.51)、(1.10±0.32)比(2.68±0.63)]和mTOR[(1.32±0.54)、(1.10±0.33)比(2.94±0.55)]mRNA表达降低(P<0.05);骨痹汤低、中、高剂量组PI3K、Akt、p-PI3K、p-Akt蛋白表达降低(P<0.05),骨痹汤中剂量组mTOR蛋白表达降低(P<0.05)。结论骨痹汤可改善骨关节炎模型大鼠膝关节的红肿程度,降低其血清炎性细胞因子水平,其作用可能与PI3K/Akt/mTOR信号通路有关。Objective To explore the effect of Gubi Decoction on serum related inflammatory factors and PI3K/Akt/mTOR signaling pathway in osteoarthritis model rats.Methods Seventy SPF rats were randomly divided into the blank group,sham operation group,Glucosamine sulfate group,and the low,medium and high dose Gubi Decoction groups.Except the blank group and sham operation group,knee osteoarthritis animal models were prepared by the modified Hulth method in each group.On the 28th day after successful model preparation,the high,medium and low dose Gubi Decoction groups were given Gubi Decoction 24,12 and 6 g/kg by gavage respectively;glucosamine sulfate group was given glucosamine sulfate tablet suspension 3 g/L by gavage,once a day for 28 days.The levels of TNF-αand IL-1βin serum were detected by ELISA.The gene expressions of PI3K,Akt and mTOR in cartilage tissue were detected by Real-PCR.The protein expressions of PI3K,Akt,p-PI3K,p-Akt and mTOR were detected by Western blot.Results Compared with the model group,the knee joint diameter[(11.17±1.81)mm,(11.60±1.38)mm,(10.80±1.17)mm vs.(12.57±0.98)mm]of the rats in the glucosamine sulfate group and the medium and high dose Gubi Decoction groups significantly decreased(P<0.05).The content of TNF-α[(111.43±21.98)ng/L,(53.42±13.25)ng/L vs.(157.89±23.60)ng/L],IL-1β[(67.50±18.44)ng/L,(48.22±9.63)ng/L vs.(96.11±14.85)ng/L]in the medium and high dose Gubi Decoction groups significantly decreased(P<0.05),and the expression of PI3K(1.87±0.17,1.24±0.49 vs.2.19±0.47),Akt(1.50±0.51,1.10±0.32 vs.2.68±0.63),and mTOR(1.32±0.54,1.10±0.33 vs.2.94±0.55)mRNA in the medium and high dose Gubi Decoction groups significantly decreased(P<0.05).The expression of PI3K,Akt,p-PI3K,p-Akt in the low,medium and high dose Gubi Decoction groups significantly decreased(P<0.05),and the expression of mTOR in the medium dose Gubi Decoction group significantly decreased(P<0.05).Conclusion Gubi Decoction can significantly reduce the level of inflammatory factors in the serum of osteoarthritis

关 键 词：骨关节炎 骨痹汤 炎症 PI3K/Akt/mTOR信号通路 大鼠 

分 类 号：R285.5[医药卫生—中药学]