G蛋白偶联雌激素受体通过减轻大鼠肾小管上皮细胞凋亡保护肾脏缺血再灌注损伤  

GPER protects renal ischemia⁃reperfusion injury by reducing the apoptosis of renal tubular epithelial cells in rats

作　　者：闫林轩 梅霄阳 章林明 常越辰 马克涛[1] 李丽[1] 王勤章[2] 李应龙[2,3] YAN Linxuan;MEI Xiaoyang;ZHANG Linming;CHANG Yuechen;MA Ketao;LI Li;WANG Qinzhang;LI Yinglong(Shihezi University Medical School,Shihezi 832000,China;不详)

机构地区：[1]石河子大学医学院,新疆石河子832000 [2]石河子大学医学院第一附属医院,新疆石河子832000 [3]成都中医药大学附属第五人民医院,成都610000

出　　处：《实用医学杂志》2021年第10期1235-1239,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81960188)。

摘　　要：目的探讨G蛋白偶联雌激素受体(G protein⁃coupled estrogen receptor,GPER)能否减轻肾小管上皮细胞凋亡,从而保护肾脏缺血再灌注(ischemia⁃reperfusion,I/R)损伤。方法雌性去卵巢(ovariec⁃tomized,OVX)大鼠随机分为OVX组、OVX+I/R组、OVX+I/R+G1(GPER激动剂)组、OVX+I/R+G15(GPER阻断剂)+G1组。检测各组大鼠肾功能,HE染色、Paller评分评价肾组织损伤程度,观察肾组织凋亡相关蛋白的表达位置及表达水平。结果与OVX组相比,OVX+I/R组肾功能损伤明显(P<0.01),Paller评分升高(P<0.01),Bcl⁃2蛋白在肾小管上皮细胞阳性表达及肾组织的表达均减少,Caspase⁃3蛋白在肾小管上皮细胞阳性表达及肾组织的表达均增加(P<0.01);G1干预后肾功能损害减轻(P<0.01),Paller评分降低(P<0.01),Bcl⁃2蛋白的表达上升,Caspase⁃3蛋白的表达下降(P<0.01);G15组可部分逆转G1激活GPER后的保护作用(均P<0.01)。结论激活GPER可以减轻肾脏I/R损伤,其机制可能是通过调控凋亡通路实现的。Objective To investigate the effect of G protein⁃coupled estrogen receptor(GPER)on ischemia⁃reperfusion(I/R)injury.Methods Female ovariectomized(OVX)rats were randomly divided into OVX group,OVX+I/R group,OVX+I/R+G1(GPER agonist)group,OVX+I/R+G15(GPER blocker)+G1 group.The renal function of rats in each group was detected,HE staining and Paller score were used to evaluate the degree of renal tissue damage,and the expression position and expression level of renal tissue apoptosis⁃related proteins were observed.Results Compared with the OVX group,the renal function of the OVX+I/R group was significantly damaged(P<0.01),Paller score increased(P<0.01),and the positive expression of Bcl⁃2 protein in renal tubular epithelial cells and the expression of renal tissue were reduced.The positive expression of Caspase⁃3 protein in renal tubular epithelial cells and the expression of renal tissue increased(P<0.01).The renal function damage was significantly reduced after G1 intervention(P<0.01),Paller score significantly decreased(P<0.01),the expression of Bcl⁃2 significantly increased,and the expression of Caspase⁃3 protein significantly decreased(P<0.01).The protective effect of G1 activated GPER G15 group was partially reversed(all P<0.01).Conclusion Activated GPER can alleviate renal I/R injury,and its mechanism may be achieved by regulating the apoptosis pathway.

关 键 词：G蛋白偶联雌激素受体 肾脏缺血再灌注损伤 肾小管上皮细胞 细胞凋亡 

分 类 号：R446.1[医药卫生—诊断学]