健康女性与子宫内膜异位症患者肠道菌群微生物组学研究  

A study on gut microbiome in healthy women versus patients with endometriosis

作　　者：韦雅婧 梁炎春[1] 杨如玉 雷舜天 王泽海 牛刚[1] WEI Yajing;LIANG Yanchun;YANG Ruyu;LEI Shuntian;WANG Zehai;NIU Gang(Department of Gynecology,the First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China;不详)

机构地区：[1]中山大学附属第一医院妇产科,广州510080 [2]中山大学中山医学院,广州510089

出　　处：《实用医学杂志》2021年第10期1279-1283,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金⁃面上项目(编号:82071618);中山大学大学生创新训练计划项目(编号:20201219,20201220);广东省科技创新战略专项资金(“攀登计划”专项资金)(编号:pd⁃jh2021b0020,pdjh2020b0015);2019年中山大学青年教师培育项目(编号:19ykpy73)。

摘　　要：目的探讨卵巢子宫内膜异位症、深部浸润型子宫内膜异位症患者与健康女性肠道菌群的差异并进行功能预测分析。方法收取2019-2020年期间卵巢子宫内膜异位症患者(OE组,n=14)、深部浸润型子宫内膜异位症患者(DIE组,n=12)及健康女性(Control组,n=10)的粪便,通过16S rRNA扩增技术对菌群V3⁃V4片段进行测序、结果分析和功能预测。结果组间菌群的多样性差异无统计学意义;OE组和DIE组的Negativicutes(纲)和Fusicatenibacter(属、种)显著减少,NK4A214_group(属)和Eubacteri⁃um_coprostanoligenes_group(种)显著增多。功能预测发现氨基酸代谢和ABC转运蛋白基因在OE组富集。结论子宫内膜异位症患者的肠道存在菌群失调,卵巢子宫内膜异位和深部浸润型子宫内膜异位的发展可能与氨基酸代谢和转运蛋白基因有关。Objective To investigate the differences in gut microbiome among patients with ovarian endo⁃metriosis,those with deep infiltrating endometriosis,and healthy women,and to predict the gene function of flora.Methods Feces were collected from 14 patients with ovarian endometriosis(OE group,n=14),12 with deep infiltrating endometriosis(DIE group,n=12),and healthy women(control group,n=10)during the period of 2019 to 2020.The common conserved region V3⁃V4 of intestinal flora was sequenced by 16S rRNA amplification and the sequencing results among three groups were analyzed;the gene function of microbiome was predicted.Results The diversity of the flora did not differ significantly among the three groups.Negativicutes and Fusicateni⁃bacter were significantly decreased while NK4A214_group and Eubacterium_coprostanoligenes_group were signifi⁃cantly increased in OE group and DIE group.Amino acid metabolism and ABC transporter were enriched in OE group.Conclusions Imbalance of intestinal flora was existed in patients with endometriosis.The development of ovarian endometriosis and deep infiltrating endometriosis may be related to amino acid metabolism and transporters.

关 键 词：子宫内膜异位症 肠道菌群 16S rRNA 免疫失衡 

分 类 号：R711.71[医药卫生—妇产科学]