我国兔出血症病毒2型全基因组测序与遗传进化分析  

Complete genome sequencing and genetic evolution analysis of Rabbit hemorrhagic disease virus type 2 in China

作　　者：于吉锋[1,2] 肖璐 周泷 谢晶 曹冶[1,2] 叶勇刚 李兴玉[1,2] 潘梦 叶健强[1,2] 魏甬 张先惠[1,2] 康润敏 林毅 YU Jifeng;XIAO Lu;ZHOU Long;XIE Jing;CAO Ye;YE Yonggang;LI Xingyu;PAN Meng;YE Jianqiang;WEI Yong;ZHANG Xianhui;KANG Runmin;LIN Yi(Sichuan Animal Science Academy,Chengdu 610066,China;Key Laboratory of Animal Genetics and Breeding of Sichuan Province,Chengdu 610066,China;Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]四川省畜牧科学研究院,成都610066 [2]动物遗传育种四川省重点实验室,成都610066 [3]西南民族大学,成都610041

出　　处：《黑龙江畜牧兽医》2021年第8期73-77,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：四川省财政运行专项(SASA2020CZYX006);四川省应用基础项目(2019YJ0562)。

摘　　要：为了研究兔出血症病毒2型(RHDV2)的基因组特性及遗传进化关系,试验对四川省某兔场死亡兔的组织病料进行RHDV2鉴定,采用RT-PCR方法扩增RHDV2全基因组分段序列,经DNAStar拼接获得病毒全基因组序列;采用MegAlign软件将分离株的全基因组、NSPs、VP60及VP10基因核苷酸序列和氨基酸序列与经典RHDV、RHDVa、RHDV2(RHDVb/GI.2)株进行了比对分析,并基于GenBank中44株RHDV的VP60基因构建分离株的系统发育树。结果表明:从病料组织中鉴定到RHDV2型毒株,命名为RHDV2-CHN/SC2020株,RHDV2-CHN/SC2020株与经典RHDV株、RHDVa株和RHDV2(RHDVb/GI.2)株的核苷酸序列相似性分别为84.6%~84.9%、83.4%~83.8%和92.0%~98.2%,与RHDV2型的相似性最高;RHDV2-CHN/SC2020株与荷兰株(RHDV2-NL2016)处于同一分支,亲缘关系最近。说明RHDV2已经传入我国,而遗传关系证实该病可能是从欧洲国家荷兰引种传入。In order to study the genomic characteristics and genetic evolution relationship of Rabbit hemorrhagic disease virus type 2(RHDV2)that occurred in China,the experiment was carried out to identify RHDV2 on infected tissue materials of dead rabbits in a rabbit farm in Sichuan Province.The RT-PCR method was used to amplify the complete gene sequence of RHDV2,and the complete genome sequence of the virus was obtained by DNAStar splicing;MegAlign software was used to analyze the homology between the complete genome,NSPs,VP60 gene,and VP10 gene nucleotide and amino acid sequences of the isolates and the classic RHDV,RHDVa,RHDV2(RHDVb/GI.2)strains;the phylogenetic tree of the isolates was constructed based on the VP60 genes of 44 RHDV strains in the GenBank database.The results showed that the RHDV2 strain was identified from the disease material tissue and named as RHDV2-CHN/SC2020 strain.The nucleotide homology between RHDV2-CHN/SC2020 and the classic RHDV,RHDVa and RHDV2(RHDVb/GI.2)strains were 84.6%-84.9%,83.4%-83.8%and 92.0%-98.2%,respectively,which meant the highest similarity with RHDV2.The RHDV2-CHN/SC2020 isolate and the Dutch strain(RHDV2-NL2016)existed in the same branch and were closely related.The results suggested that RHDV2 had been introduced into China,and genetic relationships had confirmed that the disease may be introduced from the Netherlands in Europe.

关 键 词：兔出血症病毒2型 全基因 VP60基因 遗传进化 防控 

分 类 号：S852.65[农业科学—基础兽医学] Q78[农业科学—兽医学]