血管介入法构建兔对比剂急性肾损伤模型的氧化应激和病理学改变  

Oxidative stress and pathological changes of contrast-induced acute kidney injury established by endovascular intervention in experimental rabbit models

作　　者：王征宇[1] 相建峰 彭志清[1] 陈亮[1] 李万斌 王永利[1] WANG Zhengyu;XIANG Jianfeng;PENG Zhiqing;CHEN Liang;LI Wanbin;WANG Yongli(Department of Interventional Radiology,Affiliated Sixth People's Hospital South Branch,Shanghai Jiao Tong University,Shanghai 201499,China)

机构地区：[1]上海交通大学附属第六人民医院南院介入科,201499

出　　处：《介入放射学杂志》2021年第4期368-373,共6页Journal of Interventional Radiology

基　　金：上海市奉贤区科委科学技术发展基金(奉科20131302);上海市奉贤区脑卒中临床诊疗中心建设项目(沪奉卫计-2017-118)。

摘　　要：目的采用血管介入方法构建对比剂急性肾损伤(CI-AKI)动物模型,观察其肾功能、氧化应激和病理学变化。方法新西兰大白兔随机分为4组:12 h CI-AKI组(n=3)、24 h CI-AKI组(n=3)、48 h CI-AKI组(n=3)和对照组(n=3)。所有实验兔均在DSA下接受经颈动脉血管内介入术。各CI-AKI组经腹主动脉内导管注入对比剂碘海醇构建兔CI-AKI模型,对照组经导管注入等量0.9%氯化钠注射液。分别于术前1 h,术后1 h、2 h、12 h、24 h(24 h、48 h CI-AKI组和对照组)和48 h(48 h CI-AKI组和对照组)检测肾功能和氧化应激反应。实验兔分别于术后12 h、24 h、48 h安乐死后检测肾组织匀浆氧化应激反应,并观察肾组织皮质和髓质病理学变化。结果各CI-AKI组兔术后12 h时血清肌酐(sCr)升高均>25%,血清尿素氮(BUN)显著升高;血清丙二醛(MDA)术后一过性下降,随后显著升高;血清髓过氧化物酶(MPO)术后显著升高,细胞总抗氧化能力(TAC)、过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)显著降低;肾组织匀浆MDA和MPO较对照组高,TAC、CAT、T-SOD均低于对照组;肾脏病理学检查显示肾小管上皮细胞出现不同程度的空泡样改变、颗粒变性、肾小管刷状缘节段性脱落和消失、线粒体肿胀和灶性空泡化及自噬体形成;病理学变化与sCr、BUN,MDA,MPO,TAC,CAT和T-SOD变化显著相关。对照组兔sCr、BUN及血清、肾组织匀浆MDA,MPO,TAC,CAT和T-SOD均无明显变化,病理学检查显示仅有个别肾小管上皮细胞发生空泡样改变和颗粒变性,部分线粒体肿胀、灶性空泡化。结论血管介入法可构建CI-AKI兔模型,其可产生氧化应激和肾损伤病理改变。氧化应激和肾脏病变与肾功能损害直接相关。Objective To establish an animal model of contrast-induced acute kidney injury(CI-AKI) by using endovascular intervention,and to observe the changes in renal function,oxidative stress and pathological manifestations.Methods Twelve New Zealand white rabbits were randomly divided into four groups:12-hour CI-AKI group(n=3),24-hour CI-AKI group(n=3),48-hour CI-AKI group(n=3)and control group(n=3).DSA-guided endovascular interventional procedure via carotid artery was carried out for each experimental rabbit of all groups.Injection of the contrast medium of iohexol through a catheter placed in the abdominal aorta was performed for the rabbits of the three CI-AKI groups to establish the CI-AKI models,while for the rabbits of control group the same amount of 0.9%sodium chloride was injected.The renal function and oxidative stress biomarkers were tested at one hour before intervention,as well as at one hour,2 hours,12 hours and 24 hours(only for 24-hour CI-AKI group,48-hour CI-AKI group and control group)and 48 hours after intervention(only for 48-hour CI-AKI group and control group).The experimental rabbits were separately sacrificed at 12 hours,24 hours and 48 hours.The oxidative stress reactions of renal tissue homogenate were checked,and the pathological changes in renal cortex and medulla were examined.Results In all three CI-AKI groups the postoperative 12-hour serum creatinine(sCr)levels were increased by more than 25%,the blood urea nitrogen(BUN)was remarkably increased;the postoperative serum malondialdehyde(MDA)showed a transient falling with subsequent striking up-rising;the postoperative serum myeloperoxidase(MPO)was significantly increased,while the total antioxidant capacity(TAC)of cells,catalase(CAT)and total superoxide dismutase(T-SOD)were prominently decreased;the levels of MDA and MPO in renal tissue homogenates were higher than those in the control group,while the levels of all TAC,CAT and T-SOD were lower than those in the control group.Pathologically,in the CI-AKI groups different degrees of vacuole-

关 键 词：对比剂急性肾损伤 动物模型 血管内介入术 肾功能 氧化应激 病理学检查 

分 类 号：R692.9[医药卫生—泌尿科学]