异藤黄酚对小鼠红白血病CB3细胞增殖和凋亡的影响  

Effects of isogarcinol on proliferation and apoptosis of mice erythroleukemia cell CB3

作　　者：邱剑飞 陈丽 杨珏 姚尧[2] 宋晶睿 饶青[2] 刘务玲 黄磊[2] 李艳梅 QIU Jian-fei;CHEN Li;YANG Jue;YAO Yao;SONG Jing-rui;RAO Qing;LIU Wu-ling;HUANG Lei;LI Yan-mei(Department of Immunology,School of Basic Medicine,Guizhou Medical University,Guiyang 550025,Guizhou Province,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang,550014,Guizhou Province,China;Zhijin Hospital of Traditional Chinese Medicine,Bijie 552100,Guizhou Province,China)

机构地区：[1]贵州医科大学基础医学院免疫学教研室,贵州贵阳550025 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014 [3]织金县中医院,贵州毕节552100

出　　处：《中国临床药理学杂志》2021年第9期1063-1065,1073,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81872772,81700169,81960546,U1812403);贵州省自然科学基金资助项目(QKHJC[2018]1409);贵州省科技计划基金资助项目(QKHZC[2019]2762,QKHPTRC[2020]5008)。

摘　　要：目的探讨异藤黄酚对小鼠红白血病CB3细胞增殖以及凋亡的影响。方法用噻唑蓝(MTT)法检测细胞增殖情况。根据MTT结果,将给药后细胞分为低、中、高3个浓度实验组(2.5,5.0,和10.0μmol·L^(-1)异藤黄酚,处理48 h);未给药细胞作为空白组。用流式细胞术检测CB3细胞凋亡的情况,蛋白质印迹法检测异藤黄酚作用于细胞后聚腺苷二磷酸核糖聚合酶(PARP)和胞活化的磷酸化胞外信号调节激酶(p-ERK)蛋白表达水平。结果异藤黄酚可显著抑制小鼠红白血病CB3细胞的增殖,半数抑制浓度(IC_(50))为(8.19±0.63)μmol·L^(-1)。空白组和低、中、高3个浓度实验组的细胞凋亡率分别为(1.30±0.22)%,(1.80±0.23)%,(2.50±0.52)%和(64.50±3.35)%。给药12 h后,这4组的PARP蛋白相对表达量分别为1.00±0.08,0.86±0.02,0.68±0.12和0.57±0.11;这4组的p-ERK蛋白相对表达量分别为1.00±0.02,0.57±0.13,0.54±0.02和0.05±0.01。上述指标:高浓度实验组与空白组比较,差异均有统计学意义(均P<0.05)。结论异藤黄酚可通过诱导CB3细胞发生凋亡,从而抑制细胞的增殖。其诱导CB3细胞凋亡可能与其调控PARP和p-ERK的表达有关。Objective To investigate the effects of isogarcinol on proliferation and apoptosis of mouse erythroleukemia CB3 cells. Methods MTT assay was used to assess the effects of isogarcinol on cell viability in CB3 cells at different time. According to MTT results, the cells were divided were three concentration experimental groups with 2.5,5.0 and 10.0 μmol·L^(-1) of isogarcinol for 48 h after treatment. The untreated cells served as blank group. Flow cytometry were used to detect apoptosis of CB3 cells. Protein expression of polyADP-ribose poly-merase(PARP)and phosphorylated extracellular regulatory protein kinase(p-ERK)in CB3 cells was detected by Western blot. Results Isogarcinol significantly inhibited the proliferation of CB3 cells with IC_(50) of(8.19±0.63) μmol·L^(-1). The apoptotic rates of blank group, and experimental-L,experimental –M,experimental-H groups were(1.30±0.22)%,(1.80±0.23)%,(2.50±0.52)% and(64.50±3.35)%.At 12 h after treatment, the relative expressions of polyADP-ribose poly-merase(PARP)in the 4 groups were 1. 00 ± 0. 08,0. 86 ± 0. 02,0. 68 ± 0. 12,and 0. 57 ± 0. 11,respectively;the relative expression levels of were1. 00 ± 0. 02,0. 57 ± 0. 13,0. 54 ± 0. 02,and 0. 05 ± 0. 01,respectively. Comparison between experimental-H group and blank group,the differences of the factors were significant( all P < 0. 05). Conclusion Isogarcinol suppressed CB3 cells proliferation by inducing apoptosis. The apoptosis-inducing effect of isogarcinol might be mediated at least partly through regulating the expression of PARP and p-ERK.

关 键 词：异藤黄酚 CB3细胞 红白血病 细胞增殖 细胞凋亡 

分 类 号：R28[医药卫生—中药学]