桂枝加葛根汤对神经根型颈椎病大鼠的作用和镇痛机制研究  

Effect of Guizhi Gegen Decoction on cervical spondylotic radiculopathy in rats and its analgesic mechanism

作　　者：陈会滨 樊炜骏 匡尧[1] 海兴华[3] 石玉生[1] CHEN Hui-bin;FAN Wei-jun;KUANG Yao;HAI Xing-hua;SHI Yu-sheng(Department of Massage,The First Affiliated Hospital of Tianjin University of traditional Chinese Medicine,Tianjin 200193,China;Department of Massage,ShenzhenHospital,Beijing University of Traditional Chinese Medicine(Longgang),Shenzhen 518116,Guangdong Province,China;Preventive Health Management Center of TheFirst Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 200193,China)

机构地区：[1]天津中医药大学第一附属医院推拿科,天津300193 [2]北京中医药大学深圳医院(龙岗)推拿科,广东深圳518116 [3]天津中医药大学第一附属医院治未病健康管理中心,天津300193

出　　处：《中国临床药理学杂志》2021年第9期1102-1106,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81603711)。

摘　　要：目的探讨桂枝加葛根汤对神经根型颈椎病(CSR)大鼠的作用和镇痛机制研究。方法按照体重将Wistar大鼠随机分为6组:假手术组、模型组、阳性对照组和低、中、高3个剂量实验组,每组10只。用颈椎插线法构建CSR大鼠模型。阳性对照组给予颈复康颗粒6 g·kg^(-1),低、中、高3个剂量实验组分别给予桂枝加葛根汤1,2,4 g·kg^(-1)灌胃,假手术组和模型组大鼠给予药液相同体积生理盐水灌胃。均连续给药14 d。造模后第3 d后,阳性对照组给予颈复康颗粒6 g·kg^(-1),低、中、高3个剂量实验组给予桂枝加葛根汤1,2,4 g·kg^(-1)灌胃,假手术组和模型组大鼠给予相同体积生理盐水灌胃。观察各组大鼠疼痛行为和步态障碍评分;酶联免疫吸附法检测血清中前列腺素E2(PGE2)和β-内啡肽(β-Ep)含量;以实时定量-PCR法检测脊髓和神经根组织中沉默信息调节因子1(SIRT1)、核转录因子-κB-p65(NF–κB-p65)和脑源性神经营养因子(BDNF)基因表达水平。结果假手术组、模型组、阳性对照组和低、中、高3个剂量实验组的游泳时间分别为(389.33±16.95),(332.75±13.96),(360±19.10),(358.86±17.97),(369.29±14.44)和(377.86±9.56)s;这6组的步态障碍评分分别为(1.11±0.33),(2.50±0.53),(1.89±0.60),(1.86±0.64),(1.57±0.53)和(1.28±0.49)分;这6组的左前肢疼痛阈值分别为(713.33±18.53),(406.5±14.93),(623.22±21.82),(620.88±26.51),(670.14±15.68)和(693.43±12.65)g;这6组的PGE2含量分别为(30.23±6.16),(76.89±8.74),(57.95±10.34),(59.94±10.52),(45.74±8.61)和(37.83±7.64)ng·L^(-1);这6组的β-Ep含量分别为(130.59±18.28),(63.56±9.69),(77.93±7.51),(85.43±6.72),(98.26±11.18)和(108.35±18.21)ng·L^(-1);这6组的SIRT1基因相对表达量分别为1.56±0.12,0.56±0.07,0.68±0.08,0.67±0.04,0.80±0.06和0.99±0.09;这6组的NF-κB-p65基因相对表达量分别为0.56±0.07,1.58±0.13,1.19±0.17,1.14±0.25,0.91±0.11和0.76±0.08;这6组的BDNF基因相对表达量分别Objective To investigate the effect and analgesic mechanism of Guizhi Gegen Decoction on cervical spondylotic radiculopathy( CSR) rats. Methods Wistar rats were randomly divided into 6 groups according to weight: sham operation group,model group,positive control group and experimental-L,experimental-M,experimental-H group,10 rats in each group. The CSR rat model was constructed by cervical vertebra insertion method. Three days after modeling,the positive control group was given Jingfukang granule( 6 g · kg^(-1)),the experimental-L,experimental-M,experimental-H group were given Guizhi Gegen Decoction( 1,2,4 g · kg^(-1)),and the sham operation group and model group were given the same volume of normal saline by gavage,for 14 d. The pain behavior and gait scores of rats in each group were observed. The contents of prostaglandin E2( PGE2) and β-endorphin( β-EP) in serum were detected by enzyme-linked immunosorbent assay. The expression levels of silent information regulator 1( SIRT1),nuclear transcription factor-κ b-p65( NF-κB-p65),brain-derived neurotrophic factor( BDNF),and tyrosine kinase receptor B( TrkB) in spinal cord and nerve root tissues were detected by real-time quantitative-PCR. Results The swimming time in the sham operation group,model group,positive control group and experimental-L,experimental-M,experimental-H groups were( 389. 33 ± 16. 95),( 332. 75 ± 13. 96),( 360 ± 19. 10),( 358. 86 ± 17. 97),( 369. 29 ± 14. 44), and( 377. 86 ± 9. 56) s;the scores of gait disorder in the 6 groups were( 1. 11 ± 0. 33),( 2. 50 ± 0. 53),( 1. 89 ± 0. 60),( 1. 86 ± 0. 64),( 1. 57 ± 0. 53),and( 1. 28 ± 0. 49) point,respectively;the pain threshold of left forelimb in the 6 groups were( 713. 33 ± 18. 53),( 406. 50 ± 14. 93),( 623. 22 ± 21. 82),( 620. 88 ± 26. 51),( 670. 14 ± 15. 68),and( 693. 43 ± 12. 65) g,respectively;the levels of PGE2 contents in the 6 groups were( 30. 23 ± 6. 16),( 76. 89 ± 8. 74),( 57. 95 ± 10. 34),( 59. 94 ± 10. 52),( 45. 74 ± 8. 61),( 37. 83 ± 7. 64)ng · L^(-1);the co

关 键 词：桂枝加葛根汤 神经根型颈椎病 疼痛因子 炎症介质 沉默信息调节因子1 核转录因子-κB-p65 

分 类 号：R28[医药卫生—中药学]