人工蝉花多糖对三黄鸡免疫功能及部分相关因子基因表达的影响  

Effects of polysaccharides from Cordyceps cicadae on immune function and molecular expression in Sanhuang chicken

作　　者：刘素贞 李瑞萍[3] 刘长国 卢立志 李国勤 田勇[4] 柴一秋[5] 姚火春[1] LIU Suzhen;LI Ruiping;LIU Zhangguo;LU Lizhi;LI Guoqin;TIAN Yong;CHAI Yiqiu;YAO Huochun(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;College of Animal Science,Wenzhou Vocational College of Science and Technology,Wenzhou,325000,China;College of Animal Science,Zhejiang A&F University,Hangzhou 311300,China;Institue of Animal Husbandry and Veterinary Medicine,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Zhejiang Institute of Subtropical Crops/Wenzhou Key Laboratory of Entomogenous Fungus Resources Research and Development,Wenzhou 325005,China)

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]温州科技职业学院动物科学学院,浙江温州325000 [3]浙江农林大学动物科技学院,浙江杭州311300 [4]浙江省农业科学院畜牧兽医研究所,浙江杭州310021 [5]浙江省亚热带作物研究所/温州市虫生真菌资源研究与开发重点实验室,浙江温州325005

出　　处：《畜牧与兽医》2021年第5期83-87,共5页Animal Husbandry & Veterinary Medicine

基　　金：浙江省公益基础研究项目(LGN19C170004)。

摘　　要：为探究蝉花多糖对放养三黄鸡禽流感疫苗免疫效果的影响,选取1日龄同日出雏三黄鸡1 500只随机分为3组,每组500只。其中:对照组饲喂基础饲粮,蝉花多糖组在基础饲粮中添加0.5 g/kg蝉花多糖(相当于添加蝉花粗多糖1.12 g/kg),蝉花菌丝体组在基础饲粮添加20 g/kg蝉花菌丝体(相当于添加蝉花粗多糖2.84 g/kg)。试验期共51 d。结果:蝉花菌丝体组51日龄时H5-Re8、H7N9抗体水平最高,且显著高于其他2组(P<0.05)。与对照组相比,蝉花多糖组白细胞介素1(IL-1)含量、27和51日龄时白细胞介素4(IL-4)含量、蝉花多糖组27日龄时干扰素-γ(IFN-γ)含量均显著升高(P<0.05);蝉花菌丝体组51日龄时IL-4含量、27日龄时IFN-γ含量显著提高(P<0.05);蝉花多糖组27、51日龄时、蝉花菌丝体组37日龄时肿瘤坏死因子-α(TNF-α)含量均显著升高(P<0.05)。蝉花多糖组、蝉花菌丝体组脾脏组织中IFN-γ、IL-4的mRNA表达量较对照组呈上升趋势(P>0.05)。试验表明在三黄鸡日粮中添加蝉花多糖、蝉花菌丝体可以提高鸡的体液免疫水平。To investigate the effects of Cordyceps cicadae polysaccharides on the immune function and immune-related gene expression of released chickens, 1 500 1-day-old Sanhuang chickens were randomly divided into three groups, 500 in each group. The control group was fed with a basal diet, the Cordyceps cicadae polysaccharides group(CIP Group) a basal diet supplemented with 0.5 g/kg cicada polysaccharide(equivalent to 1.12 g/kg of Cordyceps cicadae crude polysaccharides), and the Cordyceps cicadae mycelium group with a basal diet added with 20 g/kg Cordyceps cicadae mycelium( equivalent to 2. 84 g/kg of Cordyceps cicadae crude polysaccharides). The experiment lasted for 51 days. The results showed that the antibody levels of H5-Re8 and H7 N9 in the CC group were significantly higher than those in the control group( P<0. 05). Compared with the control group,the contents of IL-1,IL-4 at 27 and 51 days of age in the CIP group and the IFN-γ content in the CIP group at 27 days of age were significantly increased( P<0. 05);the contents of IL-4 and IFN-γ at 27 days of age in the CC group were significantly higher than those in the control group( P<0. 05);and the content of TNF-α in the CIP group was significantly higher than that in control group( P< 0. 05). The expression of IFN-γ and IL-4 mRNA in the spleen of the CIP group and the CC group was higher than that in the control group( P>0. 05). The above results suggested that the level of humoral immunity was improved by dietary supplementation of polysaccharide and mycelia of cicada for Sanhuang chickens.

关 键 词：蝉花多糖 三黄鸡 免疫 基因表达 

分 类 号：S859.3[农业科学—临床兽医学]