基于UPLC-Q-TOF-MS的肿节石斛化学成分研究  

Determination of the Compounds of Dendrobium pendulum Roxb.by UPLC-Q-TOF-MS

作　　者：王元成 韩彬 李振坚[1] 聂雪婷 杨明志[3] 王伟[1] 孙振元[1] WANG Yuan-cheng;HAN Bin;LI Zhen-jian;NIE Xue-ting;YANG Ming-zhi;WANG Wei;SUN Zhenyuan(Research Institute of Forestry,Chinese Academy of Forestry,Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Beijing 100091,China;Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China;Dendrobium Professional Committee of China Association of Traditional Chinese Medicine,Kunming 650002,China)

机构地区：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091 [2]中国科学院植物研究所,北京100093 [3]中国中药协会石斛专业委员会,昆明650002

出　　处：《中国药学杂志》2021年第9期708-714,共7页Chinese Pharmaceutical Journal

基　　金：国家重点研发计划项目(2018YFD1000403)。

摘　　要：目的对肿节石斛(Dendrobium pendulum Roxb.)茎、叶、花进行定性和定量研究。方法采用超高效液相色谱四极杆串联飞行时间质谱联用法(UPLC-Q-TOF-MS)技术,采用甲酸-乙腈为流动相,梯度洗脱,ESI,质量扫描范围:m/z 50~1000。结果分别鉴定为dendrocandin L(1)、芦丁(2)、金丝桃苷(3)、densiflorol A(4)、5-[2-(4-羟基-3-甲氧基苯基)乙基]-1,3-苯二酚(5)、芹菜素(6)、柚皮素(7)、3-[(E)-2-(5-羟基-3-甲氧基-1-环己-2,4-二烯基)乙烯基]-2,6-二甲氧基苯酚(8)、longicornuol A(9)、aloifol I(10)、coelonin(11)、isomoniliformine A(12)、denchryside A(13),包含联苄、黄酮、菲类、联菲等。肿节石斛花中以联苄类成分为主,叶中芦丁含量高达23.498μg·g^(-1)。结论3-[(E)-2-(5-羟基-3-甲氧基-1-环己-2,4-二烯基)乙烯基]-2,6-二甲氧基苯酚为首次在兰科植物中分离得到;除芦丁外,12个成分均为首次从肿节石斛中分离得到。OBJECTIVE To study the stems,leaves and flowers from Dendrobium pendulum Roxb qualitatively and quantitatively by UPLC-Q-TOF-MS.METHODS Formic acid and acetonitrile were used as mobile phase and gradient elution was conducted.ESI was used,and mass scanning was performed in the range of m/z 50-1000.RESULTS Thirteen components were isolated.They were identified as dendrocandin L(1),rutin(2),hyperoside(3),densiflorol A(4),tristin(5),apigenin(6),naringenin(7),cannabistilbeneⅡ(8),longicornuol A(9),aloifol I(10),coelonin(11),isomoniliformine A(12),denchryside A(13),including bibenzyls,flavonoids,phenanthrene,biphenanthrene,etc.The contents of bibenzyls in the flowers of D.pendulum were higher than those in the stems and leaves,and the contents of flavonoids in the leaves were higher with rutin of 23.498μg·g^(-1).CONCLUSION CannabistilbeneⅡis isolated from Orchidaceae for the first time,and 12 components are obtained in D.pendulum for the first time.

关 键 词：肿节石斛 联苄 黄酮 芦丁 3-[(E)-2-(5-羟基-3-甲氧基-1-环己-2 4-二烯基)乙烯基]-2 6-二甲氧基苯酚 超高效液相色谱四极杆串联飞行时间质谱联用法 

分 类 号：R284[医药卫生—中药学]