HPLC测定厚朴温中汤中7个成分的含量  

Determination of Seven Chemical Constituents in Houpo Wenzhong Decoction by HPLC

作　　者：董芳妮 王茜 郑冬梅 潘馨[1] 凌伟坚[1] DONG Fang-ni;WANG Qian;ZHENG Dong-mei;PAN Xin;LING Wei-jian(School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学药学院,福州350122

出　　处：《中国药学杂志》2021年第9期749-755,共7页Chinese Pharmaceutical Journal

基　　金：高校产学合作项目资助(2019Y4008);福建中医药大学校管课题项目资助(X2019011-学科)。

摘　　要：目的建立HPLC检测厚朴温中汤水提液中甘草苷、橙皮苷、山姜素、6-姜酚、乔松素、和厚朴酚与厚朴酚的含量,为厚朴温中汤的质量控制提供参考依据。方法采用色谱柱:北京慧德易SP ODS-AQ C18分析柱(4.6 mm×250 mm,5μm);流动相:甲醇(A)-乙腈(B)-水(C);梯度洗脱;流速:1.0 mL·min^(-1);柱温:30℃;检测波长:290 nm;进样量:10μL。运用HPLC法对厚朴温中汤的7个成分含量进行综合评价。结果甘草苷、橙皮苷、山姜素、6-姜酚、乔松素、和厚朴酚与厚朴酚分别在2.92~146.00、2.28~114.00、4.26~213.00、4.12~206.00、2.64~132.00、1.82~91.00和4.00~200.00μg·mL^(-1)内与峰面积呈现良好线性关系(r=0.9992~0.9999),平均加样回收率(n=6)在98.4%~99.0%之间,RSD为0.68%~1.46%之间。结论该方法学验证均符合要求,研究表明该方法稳定可靠、操作简便,可为厚朴温中汤质量控制及后期的标准汤剂工艺研究提供参考依据。OBJECTIVE To establish an HPLC method for detecting the contents of liquiritin,hesperidin,alpinetin,6-gingerol,pinocembrin,honokiol and magnolol in Houpo Wenzhong Decoction water extract,and provide a reference for the quality control of Houpo Wenzhong decoction.METHODS SP ODS-AQ C18(4.6 mm×250 mm,5μm);mobile phase:methanol(A)-acetonitrile(B)-water(C);gradient elution;flow rate:1.0 mL·min^(-1);column temperature:30℃;detection wavelength:290 nm;injection volume:10μL.The content of 7 components of Houpo Wenzhong decoction was comprehensively evaluated by HPLC method.RESULTS The calibration curves of liquiritin,hesperidin,alpinetin,6-gingerol,pinocembrin,honokiol and magnolol were in good linearity over 2.92-146.00、2.28-114.00、4.26-213.00、4.12-206.00、2.64-132.00、1.82-91.00、4.00-200.00μg·mL-1(r=0.9992-0.9999),respectively.The average recoveries(n=6)were in the range of 98.4%-99.0%and RSD were in the range of 0.68%-1.46%.CONCLUSION The methodological verification meets the requirements.The research shows that the method is stable,reliable,and easy to operate.It can provide a reference for the quality control of Houpo Wenzhong decoction and later standard decoction technology research.

关 键 词：厚朴温中汤 甘草苷 橙皮苷 山姜素 6-姜酚 乔松素 和厚朴酚 厚朴酚 

分 类 号：R284[医药卫生—中药学]