香椿子提取物对实验性小鼠胃黏膜损伤保护作用  

Protective effects of the extract from Toona sinensis seeds on gastric mucosa injury in experimental mice

作　　者：李丽华 章圣朋 秦加旭 年四辉 LI Lihua;ZHANG Shengpeng;QIN Jiaxu;NIAN Sihui(School of Pharmacy,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002

出　　处：《中国临床药理学与治疗学》2021年第5期546-551,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：安徽省自然科学研究基金面上项目(1908085MH272)。

摘　　要：目的:研究香椿子提取物对小鼠胃黏膜损伤的保护作用。方法:按200、400 mg·kg^(-1)·d^(-1)香椿子生药量给小鼠连续灌胃给药2周,采用乙醇及阿司匹林诱发胃黏膜损伤模型,通过计算胃黏膜损伤指数和损伤抑制率,测定小鼠血清中超氧化物歧化酶(SOD)、丙二醛(MDA)的水平和胃黏膜组织中内皮素^(-1)(ET-1)、前列腺E2(PGE_(2))、一氧化氮(NO)含量来探讨香椿子提取物对损伤的保护情况。结果:预防给药14 d后,香椿子能明显降低乙醇及阿司匹林诱发的胃黏膜损伤指数,两个剂量组对乙醇诱发的胃黏膜损伤抑制率分别为40.4%和47.4%,对阿司匹林诱发的胃黏膜损伤抑制率分别为38.9%和44.6%。阳性对照药西米替丁对乙醇及阿司匹林诱发的小鼠胃黏膜损伤抑制率分别为50.9%和50.2%。此外,与乙醇及阿司匹林诱导的模型动物组比较,香椿子200、400 mg/kg剂量组能升高小鼠胃黏膜组织中SOD、PGE2与NO水平,降低MDA和ET-1含量。结论:香椿子提取物对乙醇及阿司匹林所致小鼠胃黏膜损伤均具有一定的保护作用。AIM:To study protective effects of the extract from Toona sinensis seeds on gastric mucosal injury in experimental mice.METHODS:The mice were given 200 mg/kg,400 mg/kg of Toona sinensis seeds every day for 2 weeks and ethanol or aspirin was used to induce gastric mucosa injury model.The gastric mucosal injury index and injury inhibition rate were calculated,the levels of SOD,MDA in serum and the contents of ET-1,PGE_(2) and NO in gastric mucosal tissues were determined to explore protective effects of Toona sinensis seeds on mucosa injury.RESULTS:After 14 days of prevention administration,Toona sinensis seeds could significantly reduce the gastric mucosa injury index induced by ethanol and aspirin.The inhibition rate of ethanol-induced gastric mucosa injury in the two dose groups was 40.4%and 47.4%,and that of aspirin-induced gastric mucosa injury was 38.9%and 44.6%.The inhibitory rate of cimetidine on ethanol and aspirin-induced gastric mucosa injury was 50.9%and 50.2%,respectively.In addition,compared with the model animal group induced by ethanol and aspirin,the doses of 200 and 400 mg/kg of Toona sinensis seeds could increase the levels of SOD,PGE2 and NO in the gastric mucosal tissues of mice,and reduce the contents of MDA and ET-1.CONCLUSION:The extract of Toona sinensis seeds has a certain protective effect on the injury of gastric mucosa in mice caused by ethanol and aspirin.

关 键 词：香椿子提取物 乙醇 阿司匹林 胃黏膜损伤 保护作用 

分 类 号：R965.2[医药卫生—药理学]