LC-MS/MS法分析生猪异位发酵床垫料中5大类兽用抗菌药物残留及蓄积趋势  

Analysis of Residues of 5 Kinds of Antibiotics in Litters fromEctopic Pig Manure Fermentation Beds by LC-MS/MS

作　　者：周炜 郭炫君 王武强 陈勇 罗成江 沈红霞 张航俊 侯轩 张晓丽 王彬 孙冰冰[1] ZHOU Wei;GUO Xuan-jun;WANG Wu-qiang;CHEN Yong;LUO Cheng-jiang;SHEN Hong-xia;ZHANG Hang-jun;HOU Xuan;ZHANG Xiao-li;WANG Bin;SUN Bing-bing(Zhejiang Provincial Center for Animal Disease Prevention and Control,Hangzhou 311119,China;Taihe(Hangzhou)Pharmaceutical Technology Services Limited,Hanzhou 311121,China;Agriculture and Rural Bureau of Jinyun,Lishui,Zhejiang 321400,China)

机构地区：[1]浙江省动物疫病预防控制中心,杭州311119 [2]钛和(杭州)医药技术服务有限公司,杭州311121 [3]丽水市缙云县农业农村局,浙江丽水321400

出　　处：《中国兽药杂志》2021年第5期8-18,共11页Chinese Journal of Veterinary Drug

基　　金：浙江省重点科技研发项目(2018C02024)。

摘　　要：建立了生猪异位发酵床垫料中四环素类、喹诺酮类、磺胺类、酰胺醇类和大环内酯类共54种抗菌药物残留量的LC-MS/MS检测方法。经超低温冷冻粉碎后的垫料样本,费休氏-库仑滴定法测定水分,Mcllvaine-Na 2 EDTA缓冲液和乙腈提取,亲水亲脂平衡固相萃取小柱净化,LC-MS/MS法测定抗菌药物残留量,最终结果以折水后含量表示。对12家养殖场垫料样本中5大类兽用抗菌药物的残留量进行了检测。12家养殖场垫料样本中共检出25种抗菌药物,各场垫料样本抗菌药物总残留量在0.7~28 g/t之间,其中四环素类药物对总残留量的贡献率在91.3%~98.8%之间。方法具有简便、高通量、抗干扰的特点,可以满足生猪异位发酵床垫料中主要兽用抗菌药物残留检测的要求。A High performance liquid chromatography tandem mass spectrometry(LC-MS/MS)method for the determination of five kinds of antibiotics residues in litters from ectopic pig manure fermentation bed was developed,which was used for the analysis of tetracyclines(TETs),quinolones(FLQs),sulpha(SAs),amphenicols(AMPs)and macrolides(MIs)levels in litters from 12 ectopic pig manure fermentation beds.After superfreezed crushing,the moisture of litters was measured by Fischer coulometric titration.The target compounds were extracted by Mcllvaine-Na 2 EDTA and acetonitrile,and Solid phase extraction(HLB)clean-up step was followed.Finally,the antibiotics levels were detected by LC-MS/MS,and the results were expressed as moisture-free.The data shows 25 kinds antibiotics were found in 12 ectopic pig manure fermentation beds,the total residual levels in each pig manure fermentation beds were between 0.7 and 28 g/t,TETs residues were the highest,and its contribution rates of the total residual levels were between 91.3%and 98.8%in the 12 ectopic pig manure fermentation beds.

关 键 词：生猪异位发酵床 抗菌药物残留 

分 类 号：S859.83[农业科学—临床兽医学]