虎榆软膏治疗变应性接触性皮炎小鼠模型的药效学及作用机制  

Pharmacodynamics and mechanism of Huyu ointment on the mouse model of allergic contact dermatitis

作　　者：刘丹 夏笔军 文思 何皎 叶理 宫灿仪 邱苗芳 LIU Dan;XIA Bijun;WEN Si;HE Jiao;YE Li;GONG Canyi;QIU Miaofang(Dermatology Hosiptal,Southern Medical University,Guangzhou 510091,China;Guangzhou Women and Children’s Medical Center,Guangzhou 510623,China)

机构地区：[1]南方医科大学皮肤病医院,广东广州510091 [2]广州市妇女儿童医疗中心,广东广州510623

出　　处：《皮肤性病诊疗学杂志》2021年第2期99-104,共6页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：广东省中医药局科研项目(编号:20202123)。

摘　　要：目的:探讨虎榆软膏对变应性接触性皮炎(ACD)小鼠模型的疗效及抗炎机制。方法:用2,4-二硝基氟苯对C57小鼠腹部致敏,耳部和背部激发,分别建立急性ACD和慢性ACD模型。两种模型均随机分为5组:空白对照组、模型组、虎榆软膏组、阳性组、基质组。观察鼠耳组织病理学改变。急性ACD模型:比较各组耳厚度及左右耳质量差。慢性ACD模型:比较各组背部皮肤屏障功能、脾脏指数,PCR检测皮肤中炎症因子TNF-α、IL-1β、IL-6、IL-4、IL-17 mRNA的表达量。结果:急性、慢性ACD模型中:HE染色显示,与模型组相比,虎榆软膏组表皮增厚程度减轻,水肿减轻,真皮血管充血现象缓解;甲苯胺蓝染色显示,与模型组相比,虎榆软膏组肥大细胞数量减少。急性ACD模型:虎榆软膏组耳肿胀度为(0.041±0.010)mm,左右耳质量差为(0.845±0.296)mg;模型组耳肿胀度为(0.156±0.012)mm,左右耳质量差为(1.902±0.417)mg,两组存在明显差异(t值分别为16.46、4.62,P值均<0.01)。慢性ACD模型:皮肤屏障功能显示虎榆软膏组较模型组角质层水合力(SCH)升高(56.99±2.19比18.43±1.74,t=7.78,P<0.05)、透皮失水率(TEWL)降低(17.63±1.59比24.61±1.58,t=-3.12,P<0.05);脏器指数显示虎榆软膏组较模型组脾脏指数降低(0.0031±0.0005比0.0040±0.0005,t=-3.18,P<0.05)。PCR结果表明,与模型组相比,虎榆软膏组TNF-α、IL-1β、IL-6、IL-4、IL-17 mRNA表达量下降(t值分别为3.39、2.16、15.53、20.24、2.82,P值均<0.05)。结论:虎榆软膏对急慢性ACD小鼠模型均有较好的疗效,且未监测到局部不良反应。其作用机制可能与修复皮肤屏障,调控Th1、Th2免疫应答、降低炎症因子有关。Objective:To explore the effect and anti-inflammatory mechanism of Huyu ointment on the mouse model of allergic contact dermatitis.Methods:2,4-Dinitrofluorobenzene was used to sensitize the abdomen of C57 mice.The ears and back of mice were stimulated to establish acute and chronic ACD models.Mice were randomly divided into the blank control group,the model group,Huyu ointment group,the positive group and the matrix group.The histopathological changes of mouse ears were observed.Acute ACD model was used to measure ear thickness and quality of left and right ears.Chronic ACD model was used to detect back skin barrier function and spleen index.PCR measured inflammatory factors TNF-α,IL-1β,IL-6,IL-4,IL-17 mRNA expression levels in skin.Results:In the acute and chronic ACD model,HE staining showed that the Huyu ointment group could reduce the degree of epidermal thickening,reduce edema,and relieve dermal vascular congestion.The toluidine blue staining showed that the Huyu ointment group could reduce the number of mast cells.In the acute ACD model,the ear swelling degree was 0.041±0.010,the mass difference between the left and right ear was 0.845±0.296,while the ear swelling in the model group was 0.156±0.012,the mass difference between the left and right ear was 1.902±0.417,the two groups had a significant difference(t=16.46,4.62,respectively,both P<0.01).In the chronic ACD model,the SCH of Huyu Ointment group was significantly higher than that of model group(56.99±2.19 vs 18.43±1.74,t=7.78,P<0.05),while the TEWL significantly decreased(17.63±1.59 vs 24.61±1.58,t=-3.12,P<0.05),the spleen index also significantly decreased(0.0031±0.0005 vs 0.0040±0.0005,t=-3.18,P<0.05).PCR results showed that Huyu ointment group can reduce mRNA expression level of TNF-α,IL-1β,IL-6,IL-4 and IL-17(t=3.39,2.16,15.53,20.24,2.82,respectively,all P<0.05).Conclusions:Huyu ointment has a good effect on acute and chronic ACD mouse model.Its mechanism may be related to repairing the skin barrier,controling Th1,Th2 immune response,

关 键 词：虎榆软膏 变应性接触性皮炎 TNF-α IL-1Β IL-6 IL-4 

分 类 号：R285.5[医药卫生—中药学]