微波辅助提取甘蔗叶总黄酮的工艺优化及其抗氧化性  

Optimization of microwave-assisted extraction of total flavonoids from sugarcane leaves and and its antioxidant activities evaluation

作　　者：韦巧艳 李小梅 覃逸明 柳富杰 李大成 周永升 WEI Qiao-yan;LI Xiao-mei;QIN Yi-ming;LIU Fu-jie;LI Da-cheng;ZHOU Yong-sheng

机构地区：[1]广西科技师范学院食品与生化工程学院,广西来宾546199 [2]广西科技师范学院科研管理处,广西来宾546199

出　　处：《饲料研究》2021年第7期92-97,共6页Feed Research

基　　金：广西高校中青年教师基础能力提升项目(项目编号:2018KY0696);广西科技师范学院糖蔗资源绿色高效技术开发与应用青年科研创新团队(项目编号:GXKS2020QNTD01);广西糖资源工程技术研究中心培育建设项目(项目编号:桂科AD16450040);广西高校制糖工程综合技术重点实验室培育建设(项目编号:桂教科研[2016]6号)。

摘　　要：试验旨在优化甘蔗叶总黄酮的微波辅助提取工艺,研究其抗氧化活性。在单因素试验基础上,以乙醇浓度、微波功率及温度为主要影响因素,总黄酮提取量为考察指标,运用Box-Behnken响应面法对甘蔗叶总黄酮提取工艺进行优化。结果表明,最优提取工艺条件为液料比40 mL/g、乙醇浓度49%、微波时间4 min、微波功率400 W、微波温度61℃。在此条件下,总黄酮提取量可达10.628 mg/g,甘蔗叶总黄酮提取液对DPPH自由基和羟自由基的清除率分别为87.21%和88.69%,表明甘蔗叶总黄酮具有一定的体外抗氧化活性。The experiment aimed to optimize the microwave-assisted extraction technology of total flavonoids from sugarcane leaves and studied its antioxidant activity.On the basis of single factor experiment,the Box-Behnken response surface method was used to determine the optimal extraction conditions of sugarcane leaves flavonoids,with ethanol concentration,microwave power and temperature as the main influencing factors,and the total flavonoids extraction amount as the response index.The results showed that the optimal extraction conditions were 40 mL/g liquid-material ratio,49%ethanol concentration,4 min microwave time,400W power microwave extraction at 61℃,and the total flavonoids extraction amount reached 10.628 mg/g.The free radical scavenging rates of flavonoid extracts to·DPPH and·OH were 87.21%and 88.69%,respectively,which indicated that the total flavonoids of sugarcane leaves had certain antioxidant antioxidant capacity in vitro.

关 键 词：甘蔗叶 总黄酮 微波辅助 抗氧化 

分 类 号：S816.7[农业科学—饲料科学]