Editing of Rice Endosperm Plastidial Phosphorylase Gene OsPho1 Advances Its Function in Starch Synthesis  

作　　者：LIU Song SHAO Gaoneng JIAO Guiai ZHU Maodi WU Jiamin CAO Ruijie CHEN Yujuan XIE Lihong SHENG Zhonghua TANG Shaoqing 

机构地区：[1]China National Rice Research Institute,Hangzhou 310006,China

出　　处：《Rice science》2021年第3期209-211,共3页水稻科学（英文版）

基　　金：supported by the National Science Foundation of China(Grant No.3197150429).

摘　　要：OsPho1 in Zhonghua 11(ZH11)was edited using the clustered regularly interspaced short palindromic repeatsassociated endonuclease 9(CRISPR/Cas9)system.Two homozygous T1 mutants(cr-pho1-34 and cr-pho1-37)displayed a chalky endosperm with a white core,which significantly decreased 1000-grain weight.In addition,many rounded starch granules and abnormal amyloplasts were present in the central region of mutant endosperm cells with increased amylose and lipid contents,decreased total protein content,and altered physicochemical properties of starch.The OsPho1 protein is localized in chloroplasts,and quantitative real-time PCR(qRT-PCR)andβ-glucuronidase(GUS)staining indicated that OsPho1 was highly expressed in seeds at 5 d after fertilization(DAF).OsPho1 mutations displayed close relationships with plastidial phosphoglucomutase and ADPGlc pyrophosphorylase based onα-D-glucose-1P at different temperatures.Moreover,the expressions of starch metabolismrelated genes were also altered in the mutant,and the overexpression of OsPho1 may cause grain chalkiness.

关 键 词：CRISPR/Cas9 Pho STARCH 

分 类 号：S511[农业科学—作物学]