妊娠末期吗啡暴露的子代鼠NR2A亚基与成瘾性的关系  

Relationship of NR2A subunit and addiction in offspring rat exposed to morphineat the end of pregnancy

作　　者：杨柳[1] 张超 陈永权[1] 姚卫东[1] 鲁美静[1] 张恺辰 金孝岠[1] YANG Liu;ZHANG Chao;CHEN Yong-quan;YAO Wei-dong;LU Mei-jing;ZHANG Kai-chen;JIN Xiao-ju(Department of Anesthesiology of Yijishan Hospital,Wannan Medical College;Department of Imaging of Yijishan Hospital,Wannan Medical College,Wuhu,Anhui 241000)

机构地区：[1]皖南医学院弋矶山医院麻醉科 [2]皖南医学院弋矶山医院影像科,安徽芜湖241000

出　　处：《赣南医学院学报》2021年第4期344-348,共5页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：皖南医学院中青年科研基金项目(WK2018F08)。

摘　　要：目的:探讨妊娠末期吗啡暴露的子代鼠在不同成瘾性时脑伏隔核N-甲基-D-天冬氨酸(NMDA)受体NR2A亚基水平。方法:孕SD大鼠36只随机分为吗啡暴露组(M组)和对照组(N组),M组孕鼠第一天给予吗啡用量为2 mg·kg^(-1),此后每天增加1 mg·kg^(-1)至6 mg·kg^(-1)维持至分娩,N组孕鼠以同样方式注射等量生理盐水。取两组子代鼠50只,正常喂养至4周龄。各组子代鼠于颈背部持续皮下注射吗啡7 d,每天1次,每次3 mg·kg^(-1)进行条件位置偏爱(CPP)训练,CPP训练结束24 h后进行成瘾检测。根据CPP差值(CPP测试值-CPP基数值)将吗啡暴露组子代鼠分为高成瘾组(n=10)、中成瘾组(n=30)、低成瘾组(n=10)。采用Western blotting方法检测高、低成瘾组和对照组子代鼠伏隔核区NR2A亚基的表达。结果:(1)预测试时,各组CPP基数值的差异无统计学意义(P>0.05);高成瘾组CPP差值(552.60±132.60)s高于低成瘾组(223.40±55.14)s和对照组(42.93±31.78)s,差异均有统计学意义(P<0.01)。(2)Western blotting结果示高成瘾组的NR2A亚基表达量(0.71±0.05)高于低成瘾组(0.54±0.04)和对照组(0.22±0.04),差异有统计学意义(P<0.01)。结论:妊娠末期吗啡暴露的子代鼠成瘾性增强可能与伏隔核的NR2A亚基水平上调相关。Objective:To investigate the level of NR2A subunit of NMDA receptor in the nucleus accumbens of mor⁃phine-exposed offspring rats at the end of pregnancy during different addictions.Methods:A total of 36 late pregnant SD rats were randomly divided into morphine group(M group)and saline group(N group).Pregnant rats of M group were given morphine 2 mg·kg^(-1)on the first day and then increased by 1 mg·kg^(-1)to 6 mg·kg^(-1)every day until delivery.Pregnant rats of N group were injected with the same volume of saline in the same way.Fifty offspring of the two groups were fed normally until 4 weeks of age.The offspring of each group were subcutaneously injected with morphine on the neck and back for 7 days,once a day,3 mg·kg^(-1)g each time for CPP training.Addiction test was conducted 24 hours after the end of CPP training.The experimental rats were re-classified into three groups according to the numerical value of the CPP high preference group(n=10),moderate preference group(n=30)and low preference group(n=10).Using Western blotting method to estimate NR2A subunit expression in nucleur accumben(NAc)of high,low preference group and N group offspring rats.Results:(1)No significant difference of pretest scores staying at the non-preference chamber exist among the three groups(P>0.05).However,morphine CPP score(552.60±132.60)s of high preference group was higher than that of low preference(223.40±55.14)s and N group(42.93±31.78)s,and the differences were statisti⁃cally significant(P<0.01).(2)Western blotting showed that NR2A subunit expression in high preference group(0.71±0.05)were higher than that of low preference(0.54±0.04)and N group(0.22±0.04)and the difference was statistically significant(P<0.01).Conclusions:Increased addiction in offspring mice exposed to morphine at the end of pregnancy maybeassociated with upregulated NR2A subunit levels in the nuclens accumbens.

关 键 词：妊娠末期 吗啡 条件位置偏爱 N-甲基-D-天冬氨酸受体 NR2A亚基 成瘾性 

分 类 号：R338.64[医药卫生—人体生理学]