小麦B-box基因家族全基因组鉴定与表达分析  

Genome-wide identification and expression analysis of B-box gene family in wheat

作　　者：王艳朋 凌磊 张文睿 王丹[1] 郭长虹[1] WANG Yan-Peng;LING Lei;ZHANG Wen-Rui;WANG Dan;GUO Chang-Hong(Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province/College of Life Science and Technology,Harbin Normal University,Harbin 150025,Heilongjiang,China)

机构地区：[1]哈尔滨师范大学生命科学与技术学院/黑龙江省分子细胞遗传与遗传育种重点实验室,黑龙江哈尔滨150025

出　　处：《作物学报》2021年第8期1437-1449,共13页Acta Agronomica Sinica

基　　金：国家重点基础研究发展计划(973计划)前期研究计划项目(2011CB111500);哈尔滨师范大学研究生创新基金项目(HSDSSCX2020-08)资助。

摘　　要：B-box(BBX)是一类含有1个或2个B-box结构域的锌指蛋白,在植物生长发育中起着重要作用。本研究明确小麦B-box转录因子的数量、基因结构和分类进化关系,研究各基因成员在不同组织中的特异性表达以及对非生物胁迫的响应。从小麦全基因组中鉴定得到87个B-box基因家族成员,所有TaBBXs蛋白均含有B-box结构域。TaBBXs编码146~489个氨基酸,理论等电点为4.32~10.42。染色体定位分析表明,TaBBXs分布在除1A、1B和1D之外的18条小麦染色体上。通过系统发育分析将TaBBXs划分为5个亚家族,有0~4个内含子。在同组内同一个系统进化树分支中的亚族成员具有高度相似的基因结构。qRT-PCR分析的20个TaBBXs基因,具有不同的组织表达模式,16个基因在叶中有较高表达,TaBBX10和TaBBX39仅在叶中有较高表达,而TaBBX74在穗中表达,TaBBX43在根中特异性表达。在不同逆境胁迫下,TaBBXs呈现不同表达模式,11个基因在低温胁迫后上调表达,12个基因在ABA处理后下调表达,盐胁迫后10个基因出现上调表达,干旱胁迫后7个基因出现下调表达,TaBBX10、TaBBX39、TaBBX60、TaBBX67和TaBBX74基因在2种或2种以上胁迫下有显著的上调表达。B-box(BBX)is a class of zinc finger proteins that contain one or two B-box domains and play important roles in plant growth and development.The number,gene structure and phylogenetic relationship of wheat B-box transcription factors,as well as their expression specificity in different tissues and response to abiotic stress were investigated.A total of 87 members of B-box gene family were identified from wheat genome and all contained the B-box domain.TaBBXs encoded 146 to 489 amino acids and the isoelectric points ranged from 4.32 to 10.42.Chromosome mapping showed that these genes were distributed on 18 wheat chromosomes except 1A,1B,and 1D.Based on phylogenetic analysis,TaBBXs were divided into five subfamilies,with 0–4 introns.The members of the subfamily in the same phylogenetic tree branch in the same group had highly similar gene structures.The qRT-PCR revealed that the investigated 20 genes had different expression patterns,and most genes were highly expressed in leaves,and TaBBX10 and TaBBX39 were only highly expressed in leaves,while TaBBX74 was expressed in spikes,TaBBX43 was specifically expressed in roots.These genes showed different expression patterns under different stress.11 genes were up-regulated after low temperature stress,13 genes were down-regulated after ABA treatment,10 genes were up-regulated after salt stress,and 7 genes were down-regulated after drought stress.TaBBX10,TaBBX39,TaBBX60,TaBBX67,and TaBBX74 were significantly up-regulated under two or more stresses.

关 键 词：小麦 全基因组 B-box基因家族 非生物胁迫 基因表达 

分 类 号：S512.1[农业科学—作物学]