盐胁迫对野菊和神农香菊及其杂交F1代光合生理的影响  

Effects of Salt Stress on Photosynthetic Physiology of Chrysanthemum indicum, C. indicum var. aromaticum and Their Hybrid F Generation

作　　者：刘筱玮 夏斌 陈斌 杨扬[1] 何淼[1] Liu Xiaowei;Xia Bin;Chen Bin;Yang Yang;He Miao(Northeast Forestry University,Harbin 150040,P.R.China)

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2021年第5期32-39,共8页Journal of Northeast Forestry University

基　　金：中央高校基本科研业务费专项资金项目(2572017BA08);国家重点研发计划项目(2018YFD1000406)。

摘　　要：为探究野菊(Chrysanthemum indicum)、神农香菊(Chrysanthemum indicum var.aromaticum)及其杂交F1代5个优良株系(Z1、Z2、Z3、Z4、Z5)在盐胁迫下的光合生理特性,利用NaCl模拟4种盐浓度梯度(0、100、200、400 mmol·L^(-1))对父母本以及5个F1代株系幼苗进行胁迫处理,测定光合色素、光合参数、叶绿素荧光参数,并对其相关指标进行主成分分析和隶属函数分析。结果表明:随着胁迫浓度的增大,野菊的净光合速率(Pn)、气孔导度(Gs)、PSⅡ实际光化学效率(ΦPSⅡ)呈先上升后下降趋势,神农香菊及F1代均逐渐降低;父母本及F1代的叶绿素a质量分数、叶绿素b质量分数、叶绿素(a+b)质量分数、蒸腾速率(Tr)、潜在光化学效率(Fv/F0)、最大光化学效率(Fv/Fm)、光化学淬灭系数(qP)随着盐胁迫浓度的升高而不断下降,水分利用效率(WUE)、非光化学淬灭系数(NPQ)先上升后下降,胞间二氧化碳摩尔分数(Ci)先下降后上升。Pn与叶绿素a质量分数、叶绿素b质量分数、叶绿素(a+b)质量分数、Gs、Tr、Fv/Fm、Fv/F0、ΦPSⅡ呈极显著正相关(P<0.01),与WUE、qP、NPQ呈显著正相关(P<0.05),与Ci呈显著负相关(P<0.05)。主成分分析结果表明,相同植株对不同浓度的NaCl的反应方式大致相同,但程度上有差别;父母本及不同F1代株系对NaCl的响应方式不同。隶属函数综合评价结果表明,父母本及F1代的耐盐性由强到弱依次排名为野菊、Z2、Z1、Z3、Z4、Z5、神农香菊。综上,野菊与神农香菊的杂交F1代具有一定的耐盐能力,它们的耐盐性均优于母本,表明通过杂交手段可获得具有良好耐盐能力的芳香菊花新品种。To explore the photosynthetic physiological characteristics of Chrysanthemum indicum, C. indicum var. aromaticum and their five excellent strains(Z1, Z2, Z3, Z4 and Z5) of hybrid F1 generation in response to salt stress, NaCl was used to simulate four salt concentration gradients(0, 100, 200 and 400 mmol·L^(-1)) for stress treatment of parents and five F1 generation seedlings, then determine the photosynthetic pigments, photosynthetic parameters, chlorophyll fluorescence parameters, and their relevant indicatorsby principal component analysis and membership function analysis. The results show that with increasing stress concentration, the net photosynthetic rate(Pn), stomatal conductance(Gs), and PSⅡ actual photochemical efficiency(ΦPSⅡ) of C. indicum showed an upward trend and then decreased, and the indicators of C. indicum var. aromaticum and the F1 strains were gradually reduced. With the increase of salt concentration, chlorophyll mass a fraction, chlorophyll mass b fraction, chlorophyll mass fraction, transpiration rate(Tr), potential photochemical efficiency(Fv/F0), maximum photochemical efficiency(Fv/Fm), photochemical quenching the coefficient(qP) kept dropping. Water use efficiency(WUE) and non-photochemical quenching coefficient(NPQ) increased first and then decreased, and the intercellular carbon dioxide concentration(Ci) first decreased and then increased. The Pn of plants was significantly positively correlated with their chlorophyll mass a fraction, chlorophyll mass b fraction, chlorophyll mass fraction, stomatal conductance(Gs), transpiration rate(Tr), maximum photochemical efficiency(Fv/Fm), potential photochemical efficiency(Fv/F0), PSⅡ actual photochemical efficiency(ΦPSⅡ)(P<0.01), and significantly positively correlated with water use efficiency(WUE), non-photochemical quenching the coefficient(qP), and photochemical quenching coefficient(NPQ)(P<0.05), which was significantly negatively correlated with Ci(P<0.05). By principal component analysis, the same plants responded to NaCl of

关 键 词：菊花 NACL胁迫 光合作用 叶绿素荧光 

分 类 号：S682.11[农业科学—观赏园艺] Q945.78[农业科学—园艺学]