胡芦巴醇提取物对缓解小鼠体力疲劳的作用  

Effect of Fenugreek alcohol extract on the relieving on physical fatigue in mice

作　　者：闫冬[1] 连军[2] 闫瑶[3] 吕晓敏[2] 李琳琳[1] YAN Dong;LIAN Jun;YAN Yao;LüXiao-min;LI Lin-lin(College of Pharmacy,Xinjiang Medical University,Urumqi 830011,Xinjiang Uyghur Autonomous Region,China;Laboratory Animal Centre,Xinjiang Medical University,Urumqi 830011,Xinjiang Uyghur Autonomous Region,China;The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,Xinjiang Uyghur Autonomous Region,China)

机构地区：[1]新疆医科大学,药学院,新疆乌鲁木齐830011 [2]新疆医科大学,实验动物中心,新疆乌鲁木齐830011 [3]新疆医科大学第一附属医院,新疆乌鲁木齐830011

出　　处：《中国临床药理学杂志》2021年第10期1180-1183,共4页The Chinese Journal of Clinical Pharmacology

基　　金：新疆维吾尔自治区自然科学基金面上基金资助项目(2018D01C171);新疆维吾尔自治区中医医院博士后科研工作站基金资助项目。

摘　　要：目的研究胡芦巴醇提取物对运动疲劳小鼠的影响。方法按体重将小鼠随机分为5组:对照组、阳性药组和低、中、高3个剂量实验组,每组15只。对照组给予灭菌纯水10 mL·kg^(-1),阳性药组给予西洋参含片625.0 mg·kg^(-1),低、中、高3个剂量实验组给予胡芦巴醇提取物82.5,165.0,330.0 mg·kg^(-1)。每日1次,连续30 d。给药后30 d,记录小鼠负重游泳时间,计算血乳酸曲线下面积AUC,按照试剂盒说明书检测血清尿素氮水平和肌糖原水平。结果对照组、3个剂量(低、中、高)实验组和阳性药组小鼠负重游泳时间分别为(10.83±1.49),(11.79±1.53),(13.58±1.56),(15.15±1.84)和(13.44±1.48) min;这5组小鼠血清尿素氮水平分别为(9.64±1.05),(8.47±1.02),(8.36±0.80),(8.50±0.87)和(7.88±1.14) mmol·L^(-1);这5组小鼠血乳酸曲线下面积(比值)分别为263.94±41.35,248.71±31.20,229.10±26.52,221.09±28.11和222.93±24.94;这5组小鼠肌糖原水平分别为(0.72±0.10),(1.17±0.35),(1.30±0.34),(1.30±0.37)和(1.02±0.15)mg·g^(-1)。阳性药组和中、高2个剂量实验组与对照组比较,小鼠的负重游泳时间均显著延长,血乳酸AUC均显著降低,差异均有统计学意义(P<0.05,P<0.01);阳性药组和低、中、高3个剂量实验组与对照组比较,尿素氮水平均显著降低,而肌糖原水平均显著升高,差异有统计学意义(P<0.05,P<0.01)。结论中、高2个剂量的胡芦巴醇提取物能有效缓解小鼠运动疲劳。Objective To study the effect of Fenugreek alcohol extract(FAE) on physical fatigue in mice. Methods The mice were randomly divided into five groups according to weight: control group(Sterilized water, 10.0 mL·kg^(-1));low, medium, high dose experimental groups(FAE 82.5, 165.0, 330.0 mg·kg^(-1)) and positive drug group(Panax Quinquefolius Buccal Tablet, 625.0 mg·kg^(-1)). Each group had 15 mice, once a day for 30 d. After 30 d administratin, the time of weight loading swimming was recorded;the AUC of blood lactic acid was calculated;Serum urea nitrogen were measured by test kit;muscle glycogen were measured by the test kit. Results The loading of swimming time in control group, three dose(low, medium, high) experimental groups and positive drug group were(10.83±1.49),(11.79±1.53),(13.58±1.56),(15.15±1.84),(13.44±1.48)min, respectively;the level of serum urea nitrogen in the five groups were respectively(9.64±1.05),(8.47±1.02),(8.36±0.80),(8.50±0.87) ( 7. 88 ± 1. 14) mmol · L^(-1),respectively;the AUC of blood lactic acid in the five groups were 263. 94 ± 41. 35,248. 71 ± 31. 20,229. 10 ± 26. 52,221. 09 ± 28. 11,222. 93 ± 24. 94,respectively;muscle glycogen in the five groups were( 0. 72 ± 0. 10),( 1. 17 ± 0. 35),( 1. 30 ± 0. 34),( 1. 30 ± 0. 37),( 1. 02 ± 0. 15) mg · g^(-1),respectively.Comparison between positive drug group,two dose( medium,high) experimental groups and control group,the loading of swimming time were prolong significantly,while AUC of blood lactic acid were deceased significantly( P < 0. 05,P < 0. 01);Comparison between positive drug group,three dose( low,medium,high) experimental groups and control group,serum urea nitrogen were decreased significantly,while muscle glycogen were significantly increased( P < 0. 05,P < 0. 01). Conclusion Medium,high dose experimental groups of FAE can effectively relieve physical fatigue in mice.

关 键 词：胡芦巴 抗疲劳 负重游泳时间 尿素氮 血乳酸 肌糖原 西洋参含片 

分 类 号：R28[医药卫生—中药学]