甘草酸联合Notch1 siRNA对肺癌细胞增殖、凋亡、迁移和侵袭的影响  

Effects of glycyrrhizin combined with Notch1 siRNA on proliferation,apoptosis,migration and invasion of lung cancer cells

作　　者：王春阳[1] 刘仁龙[2] 朱瑞冬 付双[3] 赵江洋[1] 刘露[4] 曹鸿艳[5] WANG Chun-yang;LIU Ren-long;ZHU Rui-dong;FU Shuang;ZHAO Jiang-yang;LIU Lu;CAO Hong-yan(Department of Cardiothoracic Surgery,the First Hospital of Qiqihar,Qiqihar 161000,Heilongjiang Province,China;Department of Respiratory and Critical Care,the First Hospital of Qiqihar,Qiqihar 161000,Heilongjiang Province,China;Department of Chemistry,Qiqihar Medical College,Qiqihar 161000,Heilongjiang Province,China;Drug Clinical Trial Center,the First Hospital of Qiqihar,Qiqihar 161000,Heilongjiang Province,China;Department of Oncology,the First Hospital of Qiqihar,Qiqihar 161000,Heilongjiang Province,China)

机构地区：[1]齐齐哈尔市第一医院,心胸外科,黑龙江齐齐哈尔161000 [2]齐齐哈尔市第一医院,呼吸与危重症科,黑龙江齐齐哈尔161000 [3]齐齐哈尔医学院化学教研室,黑龙江齐齐哈尔161000 [4]齐齐哈尔市第一医院,药物临床试验中心,黑龙江齐齐哈尔161000 [5]齐齐哈尔市第一医院,肿瘤内科,黑龙江齐齐哈尔161000

出　　处：《中国临床药理学杂志》2021年第10期1192-1195,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究甘草酸(GA)联合Notch1小干扰RNA(siRNA)对肺癌细胞(HCC827)增殖、凋亡、迁移和侵袭的影响。方法将HCC827细胞分为对照组(正常培养的细胞)、GA组(5.0 mmol·L^(-1) GA处理)、GA+si-NC组(5.0 mmol·L^(-1) GA处理转染si-NC的细胞)、GA+si-Notch1组(5.0 mmol·L^(-1) GA处理转染si-NC细胞)。用细胞计数(CCK-8)法、流式细胞术、Transwell实验检测各组细胞增殖、凋亡、迁移侵袭情况。结果对照组、GA组、GA+si-NC组、GA+si-Notch1组HCC827细胞活力(72 h)为1.00±0.12,0.70±0.09,0.63±0.04,0.46±0.06,迁移细胞数为136.00±8.88,60.00±8.96,66.00±6.08,34.00±3.21,侵袭细胞数为106.00±7.51,45.00±5.56,48.00±3.21,29.00±3.51,凋亡率分别为(6.31±0.83)%,(15.07±0.65)%,(14.87±0.47)%,(20.61±1.64)%,对照组与GA组比较、GA+si-NC组与GA+si-Notch1组比较,上述指标差异均有统计学意义(均P<0.05)。结论甘草酸联合Notch1 siRNA1可抑制肺癌细胞增殖、迁移和侵袭,诱导细胞凋亡。Objective To study the effects of glycyrrhizin(GA) combined with Notch1 small interfering RNA(siRNA) on proliferation, apoptosis, migration and invasion of lung cancer cells(HCC827). Methods HCC827 cells were divided into control group(normal cultured cells), GA group(5.0 mmol ·L^(-1) GA treatment), GA+ si-NC group(cells transfected with si-NC and treated with 5.0 mmol·L^(-1) GA) and GA+ si-Notch1 group(cells transfected with si-Notch1 and treated with 5.0 mmol·L^(-1) GA). Cell proliferation, apoptosis, migration and invasion were detected by CCK-8, flow cytometry and Transwell assay, respectivly. Results The viability(72 h) of HCC827 cells in control group, GA group, GA+ si-NC group, GA+ si-Notch1 group were 1.00±0.12, 0.70±0.09, 0.63±0.04, 0.46±0.06;the number of migrated cells were 136.00±8.88, 60.00±8.96, 66.00±6.08, 34.00±3.21, the number of invaded cells were 106.00±7.51, 45.00±5.56, 48.00±3.21, 29.00±3.51;apoptosis rates were(6.31±0.83)%,(15.07±0.65)%,(14.87±0.47)%,(20.61±1.64) %, the differences in the above indicators were statistically significant between control group and GA group,GA + si-NC group and GA + si-Notch1 group( all P < 0. 05). Conclusion GA combined with Notch1 siR NA1 can inhibit the proliferation,migration and invasion and induce apoptosis of lung cancer cells.

关 键 词：甘草酸 肺癌 细胞增殖 凋亡 迁移 侵袭 

分 类 号：R28[医药卫生—中药学]