HPLC法测定银杏达莫注射液中双嘧达莫含量  

HPLC Method for the Determination of Dipyridamole in the Ginkgo Leaf Extractand Dipyidamole Injection

作　　者：马海霞 杨广安[1] 李玉龙[1] 罗杰[1,2] 何珺 Ma Haixia;Yang Guang’an;Li Yulong;Luo Jie;He Jun(Tongren Polytechnic College,Tongren 554300,China;Tongren Pharmaceutical Cuisine Industry Engineering Technology Research Center,Tongren 554300,China;Biochemistry Engineering Center of Guizhou Province,Guiyang 550025,China)

机构地区：[1]铜仁职业技术学院,贵州铜仁554300 [2]铜仁市药膳产业工程技术研究中心,贵州铜仁554300 [3]贵州省生化工程中心,贵州贵阳550025

出　　处：《山东化工》2021年第9期82-84,共3页Shandong Chemical Industry

基　　金：铜仁职业技术学院民族特色药用资源开发与应用创新团队(铜职院办发【2020】118号);铜仁职业技术学院专项研究黔东民间特色药用植物调研(【2020】121号);铜仁市科技计划工程中心项目(铜市科研【2018】96号)。

摘　　要：目的:建立高效液相色谱测定银杏达莫注射液中双嘧达莫含量。方法:色谱柱:Hypersil ODS2 C 18(150 mm×4.6 mm,5μm);流动相:甲醇-0.1%磷酸二氢钠溶液(75∶25);流速:1.0 mL·min^(-1);检测波长:λ=290 nm;柱温:(30±0.1)℃;进样量20μL。结果:双嘧达莫对照品溶液在7.65~38.25μg/mL范围内峰面积与浓度线性关系良好,回归方程为:Y=7928.4X+4.879(R=0.9993),线性范围7.65~38.25μg/mL。结论:该方法操作更加简捷、方便、安全性更高,可用于测定银杏达莫注射液中双嘧达莫含量,以便于其质量控制。Objective:To establish an HPLC method for the determination of dipyridamole in the ginkgo leaf extract and dipyidamole injection.Method:The chromatographic conditions included Hypersil ODS2 C 18 column(150 mm×4.6 mm,5μm),methanol-0.1%sodium dihydrogen phosphate solution(75∶25)at the flow rate 1.0 mL·min^(-1) of the mobile phase,and at(30±0.1)℃;The injection volume is 20μL.Results:The linear range of dipyridamole was 7.65~38.25μg·mL^(-1).The regression equation was Y=7928.4X+4.879(R=0.9993).Conclusion:The HPLC method was precise,reproducible and stable,which can be applied in the quality control of ginkgo leaf and dipyridamole injection.

关 键 词：银杏达莫注射液 双嘧达莫 HPLC 

分 类 号：O657.7[理学—分析化学]