QTL mapping of seedling biomass and root traits under different nitrogen conditions in bread wheat(Triticum aestivum L.)  

作　　者：YANG Meng-jiao WANG Cai-rong Muhammad Adeel HASSAN WU Yu-ying XIA Xian-chun SHI Shu-bing XIAO Yong-gui HE Zhong-hu 

机构地区：[1]College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,P.R.China [2]National Wheat Improvement Centre,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences(CAAS),Beijing 100081,P.R.China [3]Institute of Agricultural Science of Yili Prefecture,Yining 835000,P.R.China [4]International Maize and Wheat Improvement Centre(CIMMYT)China Office,c/o CAAS,Beijing 100081,P.R.China

出　　处：《Journal of Integrative Agriculture》2021年第5期1180-1192,共13页农业科学学报（英文版）

基　　金：the National Key R&D Program of China(2016YFD0101804-6);the National Natural Science Foundation of China(31671691);the International Science&Technology Cooperation Program of China(2016YFE0108600)。

摘　　要：Plant nitrogen assimilation and use efficiency in the seedling's root system are beneficial for adult plants in field condition for yield enhancement.Identification of the genetic basis between root traits and N uptake plays a crucial role in wheat breeding.In the present study,198 doubled haploid lines from the cross of Yangmai 16/Zhongmai 895 were used to identify quantitative trait loci(QTLs)underpinning four seedling biomass traits and five root system architecture(RSA)related traits.The plants were grown under hydroponic conditions with control,low and high N treatments(Ca(NO_(3))_(2)·4H_(2)O at 0,0.05 and 2.0 mmol L^(-1),respectively).Significant variations among the treatments and genotypes,and positive correlations between seedling biomass and RSA traits(r=0.20 to 0.98)were observed.Inclusive composite interval mapping based on a high-density map from the Wheat 660 K single nucleotide polymorphisms(SNP)array identified 51 QTLs from the three N treatments.Twelve new QTLs detected on chromosomes 1 AL(1)in the control,1 DS(2)in high N treatment,4 BL(5)in low and high N treatments,and 7 DS(3)and 7 DL(1)in low N treatments,are first reported in influencing the root and biomass related traits for N uptake.The most stable QTLs(RRS.caas-4 DS)on chromosome 4 DS,which were related to ratio of root to shoot dry weight trait,was in close proximity of the Rht-D1 gene,and it showed high phenotypic effects,explaining 13.1%of the phenotypic variance.Twenty-eight QTLs were clustered in 12 genetic regions.SNP markers tightly linked to two important QTLs clusters C10 and C11 on chromosomes 6 BL and 7 BL were converted to kompetitive allele-specific PCR(KASP)assays that underpin important traits in root development,including root dry weight,root surface area and shoot dry weight.These QTLs,clusters and KASP assays can greatly improve the efficiency of selection for root traits in wheat breeding programmes.

关 键 词：KASP marker QTL analysis root traits SNP array Triticum aestivum 

分 类 号：S512.1[农业科学—作物学]