慢性鼻-鼻窦炎伴息肉发病中HIF-1α表达及对Alox5和炎症因子分泌的影响研究  

Expression of HIF-1αand its effect on secretion of Alox5 and inflammatory factors in chronic rhinosinusitis with nasal polyps

作　　者：王嘉 田东倩 魏英粉 杨泽垠 邸斌 Wang Jia;Tian Dongqian;Wei Yingfen;Yang Zeyin;Di Bin(E.N.T.Department,Hospital 980 of PLA Joint Logistics Support Force,Shijiazhuang,Hebei,050000,China)

机构地区：[1]解放军980医院耳鼻喉科,石家庄050000

出　　处：《西南国防医药》2021年第5期373-377,共5页Medical Journal of National Defending Forces in Southwest China

基　　金：河北省卫健委青年科技课题项目(20200228)。

摘　　要：目的探讨慢性鼻-鼻窦炎伴息肉发病中HIF-1α的表达及对Alox5和炎症因子分泌的影响研究。方法选取受手术治疗的26例慢性鼻-鼻窦炎伴息肉患者的标本为鼻息肉组,选择20例鼻窦炎但无鼻息肉患者的窦黏膜组织为无鼻息肉组,另选同期接受治疗的20例鼻中隔手术或鼻窦囊肿患者的钩突或中鼻甲黏膜组织作为对照组。通过苏木精-伊红染色法(HE)观察鼻息肉组织病理学情况,采用免疫组织化学染色检测中HIF-1α的表达情况,通过qRT-PCR检测炎症因子分泌情况,采用Western blot检测Alox5表达情况。结果与对照组和无鼻息肉组相比,鼻息肉组的嗜酸性粒细胞明显增多(P<0.05);鼻息肉组的HIF-1α的IOD值显著高于无鼻息肉组(P<0.05);鼻息肉组中的1L-4、1L-13、1L-17A mRNA的相对表达水平显著高于无鼻息肉组(P<0.05);siHIF-1α组中Alox5蛋白表达水平显著低于阴性对照组(P<0.05);与阴性对照组相比,siHIF-1α组中1L-4、1L-13、1L-17A mRNA的相对表达水平显著降低(均P<0.05)。结论慢性鼻-鼻窦炎伴息肉中HIF-1α呈现高表达,沉默HIF-1α,能够降低Alox5的表达和炎症因子分泌,其机制可能通过抑制Alox5来降低炎症因子的分泌。Objective To explore the expression of HIF-1αand its effect on the secretion of Alox5 and inflammatory factors in chronic rhinosinusitis with nasal polyps.Methods Specimens from 26 patients with chronic rhinosinusitis with nasal polyps were selected as the nasal polyp group,sinus mucosa from 20 patients with sinusitis without nasal polyps were selected as the non-nasal polyp group,and uncinate process or middle turbinate mucosa of 20 patients with nasal septum surgery or nasal sinus cyst receiving treatment in the same period were selected as the control group.The histopathology of nasal polyps was observed by hematoxylin-eosin(HE)staining,the expression of HIF-1αwas detected by immunohistochemical staining,the secretion of inflammatory factors was detected by qRT-PCR,and the expression of Alox5 was detected by Western blot.Results The eosinophils of the nasal polyp group significantly increased compared with the control group and the non-nasal polyp group(P<0.05);IOD value of HIF-1αin the nasal polyp group was significantly higher than that in the non-nasal polyp group(P<0.05);the relative expression levels of IL-4,IL-13 and IL-17 A mRNA in the nasal polyp group were significantly higher than those in the non-nasal polyp group(P<0.05);and the expression level of Alox5 protein in the siHIF-1αgroup was significantly lower than that in the negative control group(P<0.05).Compared with the negative control group,the relative expression levels of IL-4,IL-13 and IL-17 A mRNA in the siHIF-1αgroup significantly decreased(P<0.05).Conclusion HIF-1αis highly expressed in chronic rhinosinusitis with nasal polyps.Silencing HIF-1αcan reduce the expression of Alox5 and the secretion of inflammatory factors through the mechanism of inhibiting Alox5 to reducing secretion of inflammatory factors.

关 键 词：慢性鼻-鼻窦炎伴息肉 乏氧诱导因子 5-脂加氧酶 

分 类 号：R765.41[医药卫生—耳鼻咽喉科]