重庆市荣昌区中华田园犬蜱种鉴定及序列分析  

Identification and Sequence Analysis of Tick Species of Chinese Rural Dogs in Rongchang District of Chongqing

作　　者：陈绍基 李芳 谭纯 贾红菓 梅四鹏 周荣琼 CHEN Shao-ji;LI Fang;TAN Chun;JIA Hong-guo;MEI Si-peng;ZHOU Rong-qiong(School of Veterinary Medicine,Southwest University,Rongchang Chongqing 402460,China)

机构地区：[1]西南大学动物医学院,重庆荣昌402460

出　　处：《西南大学学报（自然科学版）》2021年第5期56-61,共6页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(31672541,31172313).

摘　　要：蜱是专性吸血的外寄生节肢动物,其种类丰富,分布广泛,可携带病原并有传播疾病风险,给人和动物健康带来威胁.本试验对重庆市荣昌区中华田园犬蜱进行形态学和16S rDNA分子生物学鉴定及进化分析.形态学鉴定结果显示荣昌区中华田园犬蜱种类为长角血蜱Haemaphysalis longicornis.对3株样本的16S rDNA基因进行扩增,测序显示其序列长度为457~458 bp,仅存在个别碱基差异.系统发育进化树显示3株样本与已经报道的H.longicornis(GeneBank NO.JX051073,KJ710087,MT555304,MN956526)位于同一个分支,同源性高达99.3%~99.6%;与豪猪血蜱H.hystricis(GeneBank NO.LT593116)的遗传距离最近,与微小牛蜱Boophilus microplus(GeneBank NO.AY974242)的遗传距离最远.Ticks are specialized blood-sucking ectoparasitic arthropods which are rich in species and widely distributed.They can carry pathogens and have the risk of spreading diseases,posing a threat to human and animal health.In this experiment,the Chinese rural dog ticks in Rongchang District of Chongqing were identified with morphological and 16S rDNA molecular biological methods,and their evolution was analyzed.The morphological identification showed that the tick species of Chinese rural dog in this area was Haemaphysalis longicornis.Then the 16S rDNA genes of three samples were amplified,and sequencing revealed that their sequence length was 457-458 bp,with differences in only a few bases.The phylogenetic evolutionary tree showed that the three samples were located in the same branch as those already reported for H.longicornis(No.JX051073,KJ710087,MT555304 and MN956526),with 99.3%to 99.6%homology;they were close to H.hystricis(No.LT593116)with the smallest genetic distance and far apart from Boophilus microplus(No.AY974242)with the greatest genetic distance.

关 键 词：中华田园犬 长角血蜱 鉴定 序列分析 

分 类 号：Q959.22[生物学—动物学]