一株猪源化脓隐秘杆菌的分离鉴定及对促炎细胞因子分泌的影响  

Isolation and Identification of Trueperella pyogenes from Swine and Its Effect on the Secretion of Proinflammatory Cytokines

作　　者：田尚全 王近礼 易文毅 谭静梅 冉金鑫 王芝英 周作勇[1,2] TIAN Shang-quan;WANG Jin-li;YI Wen-yi;TAN Jing-mei;RAN Jin-xin;WANG Zhi-ying;ZHOU Zuo-yong(School of Veterinary Medicine,Southwest University,Rongchang Chongqing 402460,China;Veterinary Science Engineering Research Center of Chongqing,Rongchang Chongqing 402460,China)

机构地区：[1]西南大学动物医学院,重庆荣昌402460 [2]重庆市兽医科学工程研究中心,重庆荣昌402460

出　　处：《西南大学学报（自然科学版）》2021年第5期62-68,共7页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市技术创新与应用示范(社会民生类)项目(cstc2018jscx-msybX0216);中央高校基本科研业务费专项资金资助项目(XDJK2020B016).

摘　　要：检测了重庆市璧山区一起屠宰猪肉中出现化脓性病变样本的病原菌,并研究其药物敏感性、毒力基因分布及其对巨噬细胞分泌IL-6和TNF-α的影响.从样本中分离获得一株具有极强溶血性的菌株(BS-1株),其16S rRNA序列与GenBank中的猪化脓隐秘杆菌序列(LC500012.1)相似度最高,为97.26%.系统发育分析显示BS-1株与其他化脓隐秘杆菌处于同一分支.药敏试验显示BS-1株除对克拉霉素和罗红霉素中度敏感外,对青霉素G、庆大霉素、万古霉素、诺氟沙星、氯霉素、米诺环素(甲)和阿莫西林等耐药.毒力基因检测发现BS-1株含有Plo,NanH,NanP,FimA基因,无CbpA和FimC基因.细胞因子检测证实BS-1株能诱发巨噬细胞IL-6和TNF-α分泌水平显著增加.试验结果表明:该猪肉中出现化脓性病变的病原菌为化脓隐秘杆菌,该菌携带了多种毒力基因,对常见抗菌药物耐药性严重.This study detected the pathogenic bacteria that caused the purulent lesions in slaughtered pig pork in Bishan District,Chongqing,and studied the drug sensitivity,virulence gene distribution and the ability of the isolated bacteria that induced IL-6 and TNF-αsecretion in macrophages.In result,a strain(BS-1)with extremely strong hemolytic propertieswas isolated from the sample,and the 16S rRNA sequence of BS-1 was found to be most similar(97.26%)to that of Trueperella pyogenes(LC500012.1),and the phylogenetic analysis showed that BS-1 was located in the same branch as the other T.pyogenes strains.The drugsusceptibility test showed that BS-1 was moderately sensitive to clarithromycin and roxithromycin,but resistant to penicillin G,gentamicin,vancomycin,norfloxacin,chloramphenicol,minocycline(A)and amoxicillin.irulence gene test showed that BS-1 contained Plo,NanH,NanP,and FimA,but no CbpA and FimC.Cytokine test confirmed that BS-1 induced a significant increase in IL-6 and TNF-αsecretion in macrophages.These results indicated that the pathogen of pyogenic lesions in the slaughtered pork was T.pyogenes,which carried multiple virulence genes and was highly resistant to common antimicrobial drugs.

关 键 词：猪 化脓隐秘杆菌 16S rRNA 毒力基因 

分 类 号：S828[农业科学—畜牧学]