新生儿1,25-二羟基维生素D_(3)与CMVI及预后的关系  

Relationship between serum 1,25-dihydroxyvitamin D_(3)with CMVI and prognosis in neonates

作　　者：邱锐琴[1] 夏明月[2] 韩雁雁 杜志云[1] 姚文秀[1] QIU Ruiqin;XIA Mingyue;HAN Yanyan;DU Zhiyun;YAO Wenxiu(Department of Neonatalogy,Qinhuangdao Municipal First Hospital,Qinhuangdao,Hebei 066000,China;Department of Pediatrics,Qinhuangdao Municipal First Hospital,Qinhuangdao,Hebei 066000,China)

机构地区：[1]河北省秦皇岛市第一医院新生儿科,066000 [2]河北省秦皇岛市第一医院儿科,066000

出　　处：《重庆医学》2021年第10期1729-1732,1736,共5页Chongqing medicine

基　　金：河北省秦皇岛市自筹经费项目(201902A130)。

摘　　要：目的探讨新生儿血清1,25-二羟基维生素D_(3)[1,25-(OH)_(2)D_(3)]水平与巨细胞病毒感染(CMVI)及预后的关系。方法选取2016年3月至2019年7月于该院就诊的60例初发CMVI新生儿为研究对象(CMVI组),选取同期75例健康新生儿作为对照组,随访后根据CMVI新生儿预后情况,将其分为好转或痊愈组和异常或死亡组。采用酶联免疫吸附试验(ELISA)法检测血清1,25-(OH)_(2)D_(3)、白细胞介素17(IL-17)、转化生长因子β1(TGF-β1)水平,流式细胞仪检测γδT细胞和Treg细胞表达水平;分析CMVI新生儿1,25-(OH)_(2)D_(3)与γδT、Treg及IL-17、TGF-β1水平的相关性及影响CMVI新生儿预后的因素。结果CMVI组γδT、IL-17水平高于对照组,1,25-(OH)_(2)D_(3)、Treg、TGF-β1水平低于对照组,差异均有统计学意义(P<0.05);CMVI新生儿1,25-(OH)_(2)D_(3)水平与Treg、TGF-β1呈正相关(r=0.532、0.498,P=0.002、0.012),与γδT、IL-17呈负相关(r=-0.512、-0.476,P=0.008、0.016);异常或死亡组γδT、IL-17水平高于好转或痊愈组,1,25-(OH)_(2)D_(3)、Treg、TGF-β1水平低于好转或痊愈组,差异均有统计学意义(P<0.05);γδT是CMVI新生儿好转或痊愈的危险因素,1,25-(OH)_(2)D_(3)是CMVI新生儿好转或痊愈的保护因素。结论血清1,25-(OH)_(2)D_(3)与新生儿CMVI密切相关,可能对评估预后有重要意义。Objective To investigate the relationship between the serum 1,25-dihydroxyvitamin D_(3)[1,25-(OH)_(2)D_(3)]level with cytomegalovirus infection(CMVI)and prognosis in neonates.Methods Sixty newborn infants with CMVI in this hospital from March 2016 to July 2019 were selected as the study subjects(CMVI group),and at the same time 75 healthy newborns were selected as the control group.After follow-up,according to the prognosis situation of CMVI newborns,they were divided into the improvement or cure group and death group.The levels of serum 1,25-(OH)_(2)D_(3),interleukin-17(IL-17)and transforming growth factor-β1(TGF-β1)were detected by adopting the enzyme-linked immunosorbent assay(ELISA),and the expression levels ofγδT cells and Treg cells were detected by flow cytometry;the correlations between 1,25-(OH)_(2)D_(3)andγδT,Treg,IL-17 and TGF-β1 levels in CMVI newborns were analyzed.Results The levels ofγδT and IL-17 in the CMVI group were higher than those in the control group,the levels of 1,25-(OH)_(2)D_(3),Treg and TGF-β1 were lower than those in the control group,and the differences were statistically significant(P<0.05);the 1,25-(OH)2D_(3)level in the neonates with CMVI was positively correlated with the Treg and TGF-β1 levels(r=0.532,0.498,P=0.002,0.012),and negatively correlated with theγδT and IL-17 levels(r=-0.512,-0.476,P=0.008,0.016);the levels ofγδT and IL-17 in the abnormality or death group were higher than those in the improvement or cure group,while the levels of 1,25-(OH)_(2)D_(3),Treg and TGF-β1 were lower than those in the improvement or cure group,and the edifferences were statistically significant(P<0.05);γδT was a risk factor for the improvement or cure of CMVI newborns,and 1,25-(OH)_(2)D_(3)was a protective factor for the improvement or cure of CMVI newborns.Conclusion Serum 1,25-(OH)_(2)D_(3)is closely correlated to CMVI in neonates,which may be of great significance in evaluating the prognosis prognosis.

关 键 词：新生儿 血清 1 25-二羟基维生素D_(3) 巨细胞病毒感染 预后 

分 类 号：R722.1[医药卫生—儿科]