Box-Behnken响应面法结合差示分光光度法优化瓜蒌中总黄酮提取工艺  

Optimization of the extraction process of total flavonoids from Trichosanthis Fructus by Box-Behnken response surface method combined with differential spectrophotometry

作　　者：胡倩 钱婉智 邹纯才 鄢海燕 HU Qian;QIAN Wan-zhi;ZOU Chun-cai;YAN Hai-yan(Pharmacy School of Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002

出　　处：《海南医学院学报》2021年第11期834-839,共6页Journal of Hainan Medical University

基　　金：安徽高校省级自然科学研究重大项目(KJ2016SD60,KJ2015ZD41)。

摘　　要：目的:优化瓜蒌(由瓜蒌皮和瓜蒌子按一定比例组成)中总黄酮的提取工艺。方法:考察瓜蒌皮和瓜蒌子的比例、乙醇浓度、超声提取时间和提取温度对瓜蒌中总黄酮提取率的影响,并采用Box-Behnken响应面法结合差示分光光度法优化瓜蒌中总黄酮的提取工艺。结果:瓜蒌中总黄酮的最佳提取工艺为:瓜蒌皮和瓜蒌子的比例为4∶6、乙醇浓度为70%、超声提取时间为60 min和提取温度为40℃。结论:BoxBehnken响应面法结合差示分光光度法较好地优化了瓜蒌中总黄酮的的提取工艺,可为瓜蒌中总黄酮的提取应用提供参考。Objective:To optimize the extraction process of total flavones in Trichosanthis Fructus(composed of Trichosanthis pericarpium and semen Trichosanthis in a certain proportion).Methods:The effects of the mixture ratio of Trichosanthis pericarpium and semen Trichosanthis,ethanol concentration,ultrasonic extraction time and extraction temperature on the extraction rate of total flavonoids in Trichosanthis Fructus were investigated.The extraction process of total flavonoids in Trichosanthis Fructus was optimized by Box-Behnken response surface method combined with differential spectrophotometry.Results:The optimum extraction conditions of total flavonoids in Trichosanthis Fructus were as follows:The mixture ratio of Trichosanthis pericarpium and semen Trichosanthis was 4∶6,the ethanol concentration was 70%,the ultrasonic extraction time was 60 min and the extraction temperature was 40℃.Conclusion:Box-Behnken response surface method combined with differential spectrophotometry can optimize the extraction process of total flavonoids from Trichosanthis Fructus,which can provide a reference for the extraction and application of total flavonoids in Trichosanthis Fructus.

关 键 词：瓜蒌皮 瓜蒌子 总黄酮 提取工艺 Box-Behnken响应面法 

分 类 号：R284.2[医药卫生—中药学]