后置反硝化的设置对低温下碳源调控-回收磷工艺系统(BBNR-CPR)脱氮除磷的影响  

Effect of post-denitrifying setup on nitrogen and phosphorus removal in the biofilm bio-nutrients removal-carbon source regulated phosphorus recovery process(BBNR-CPR)at low temperature

作　　者：林欢 焦彭博 田晴 蒋涛[1,2] 董雪彤 朱艳彬 李方 杨波[1,2] LIN Huan;JIAO Pengbo;TIAN Qing;JIANG Tao;DONG Xuetong;ZHU Yanbin;LI Fang;YANG Bo(State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry,Shanghai 201620,China;College of Environmental Science and Engineering,Donghua University,Shanghai 201620,China)

机构地区：[1]国家环境保护纺织工业污染防治工程技术中心,上海201620 [2]东华大学环境科学与工程学院,上海201620

出　　处：《环境工程学报》2021年第4期1260-1269,共10页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(21777024);上海自然科学基金资助项目(16ZR1402000);国家重点研发计划(2019YFC0408503)。

摘　　要：采用连续运行式生物膜脱氮蓄磷-碳源调控回收磷系统(biofilm bio-nutrient removal carbon source regulated phosphorus removal,BBNR-CPR)处理低C/N比(3.4~6.9)模拟生活污水。通过反应器内生物膜来蓄积废水中的磷,同时采用周期性投加高浓度的外加碳源,诱导释放生物膜内蓄积的磷且对其进行回收。在此基础上,通过增设后置缺氧段,同时增加好氧内循环量、提高磷回收阶段补充碳源浓度等方式,强化BBNR-CPR系统的运行,以期实现低温下(<15℃)系统的同步脱氮蓄磷/回收磷的目标。结果表明,在低温下引入后置缺氧段,可节省27%的曝气能耗,并能维持该系统脱氮除磷性能的稳定性。在进水15 mg·L^(-1)的条件下,该系统对NH4+-N、TN和TP的平均去除率分别达到了89.12%、82.14%和89.24%。在单个生物蓄磷-磷回收周期(7d)内,随着系统运行时间的延长(第3~6天),生物膜内反硝化聚磷菌体内的PHA的不断消耗,系统的缺氧吸磷速率仍可维持稳定,第3和6天分别为7.51 mg·(L·h)^(-1)和7.83 mg·(L·h)^(-1))。在该运行方式下,系统后置缺氧段每去除1.00 mg NO3--N可耦合去除0.76 mg TP;且该阶段限制反硝化除磷的主要因素是进水氨氮转化时产生的硝态氮(反硝化吸磷电子受体)的浓度。通过对生物膜样本进行16S rRNA高通量测序分析,发现系统内的优势菌群为Candidatus Competibacter、Candidatus Nitrotoga、Phaeodactylibacter、Thiothrix和Dechloromonas。A continuous biofilm bio-nutrient removal-carbon source regulated phosphorus removal(BBNRCPR)process was used as the alternative anaerobic/aerobic and alternative anaerobic/aerobic/anoxic biofilter to treat the simulated municipal wastewater with low C/N ratio(influent C/N ratio from 3.44 to 6.91).Phosphorus(P)in the wastewater was accumulated in the biomass,and at the same time,a high concentration of external carbon source was periodically introduced to release the accumulated P in the biofilm for further recovery.On this basis,the BBNR-CPR system was enhanced to achieve the goal of simultaneous P storage/removal at low temperature during aerobic and post-denitrification phase,which was achieved by the post-anoxic phase setup,the increase of the internal circulation during aerobic phase and the supplementary carbon source amount during periodical P-harvesting phase under anaerobic conditions.The results showed that the system could save 27%of energy supply by introducing a post anoxic phase,and maintain a stable nitrogen and phosphorous removal performance at low temperatures(<15℃).Meanwhile,the average removal rates of NH4+-N,TN and TP reached89.12%,82.14%and 89.24%,respectively,at the influent NH4+-N of 50 mg·L^(-1)and TP of 15 mg·L^(-1).During a P accumulation/recovery cycle(7 days),the PHA storage amount in the biomass of denitrification phosphorous accumulation organisms(DPAOs)was continuously consumed with the extension of the biofilter operating time(day 3-day 6),while the rate of denitrifying P uptake maintained stable(7.51 mg(L·h)^(-1)on the 3 rd day,7.83 mg(L·h)^(-1)on the 5 th day).The post-denitrifying phase could removal 0.76 mg TP when it removed 1.00 NO3--N.The main limiting factor of denitrifying phosphorus removal at this stage was the availability of nitrate nitrogen(as the electron acceptor for denitrifying phosphorus uptake)produced during ammonia nitrogen transformation.16 S rRNA high-throughput sequencing analysis of biofilm samples showed that the dominate functional communities in

关 键 词：低温 低C/N比 同步脱氮除磷 后置反硝化除磷 生物膜 

分 类 号：X703.1[环境科学与工程—环境工程]