碳氮比对高盐废水单室MFCs产电、污染物去除及微生物群落结构的影响  

Effects of carbon-nitrogen ratio on electricity generation,pollutant removal and microbial community structure of single-chamber MFCs in high salinity wastewater

作　　者：王佳琪 付国楷[1] 黄梓良 何宇轩 黎诗语 刘银 WANG Jiaqi;FU Guokai;HUANG Ziliang;HE Yuxuan;LI Shiyu;LIU Yin(Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment,Ministry of Education,College of Environment and Ecology,Chongqing University,Chongqing 400045,China;Chongqing Yucai Secondary School,Chongqing 400050,China)

机构地区：[1]重庆大学环境与生态学院,三峡库区环境与生态部重点实验室,重庆400045 [2]重庆育才中学,重庆400050

出　　处：《环境工程学报》2021年第4期1354-1366,共13页Chinese Journal of Environmental Engineering

基　　金：重庆市自然科学基金资助项目(cstc2019jcyj-msxmX0573);重庆市青少年创新人才培养雏鹰计划(CY200147)。

摘　　要：构建了5套单室无膜空气阴极微生物燃料电池(microbial fuel cell,MFC),探讨了碳氮比对单室MFC产电及污染物去除效果的影响,并通过微生物高通量测序分析了电极生物膜的优势菌种。结果表明:当碳氮比分别是3∶1、4∶1、5∶1、6∶1和7∶1时,在产电性能方面,碳氮比的提高有利于电池电能的输出;当碳氮比为7∶1时,开路电压、内阻及最大功率密度分别为765 mV、78.4Ω和7.33 W·m^(-3);单室MFCs可实现同步硝化反硝化脱氮,当碳氮比为4∶1时,污染物的去除效果最佳,COD、(96.98±1.8)%和(96.64±1.8)%。微生物测序结果表明,Thauera为单室MFCs的核心菌属,随碳氮比的升高,阴极生物膜中具有异养硝化功能的微生物丰度依次为35.72%、46.90%、40.17%、35.63%和21.38%;好氧反硝化菌的丰度分别为35.72%、52.60%、49.59%、45.08%和21.38%,由此推测,氮的去除以异养硝化-好氧反硝化途径为主。In this study,five sets of single-chambered air cathode microbial fuel cells(MFC)were constructed.The effect of the carbon-nitrogen ratio on electricity generation and pollutant removal was discussed and the dominant strains in electrode biofilms were analyzed by high-throughput sequencing.The results showed that in terms of electricity generation performance,the increase of the carbon-nitrogen ratio was beneficial to the energy output at the carbon-nitrogen ratio of 3∶1,4∶1,5∶1,6∶1 and 7∶1.When the carbon-nitrogen ratio was7∶1,the open circuit voltage,internal resistance and maximum power density were 765 mV,78.4Ωand 7.33 W·m^(-3),respectively.Single-chamber MFCs could achieve simultaneous nitrification and denitrification,and the best removal effect of pollutants occurred at the carbon-nitrogen ratio of 4∶1,the corresponding removal rates of COD,N H4+-N and TN were(86.17±2.4)%,(96.98±1.8)%and(96.64±1.8)%,respectively.Microbial sequencing results showed that Thauera was the core genus of single-chamber MFC.With the increase of carbon-nitrogen ratio,the abundances of heterotrophic nitrification microorganisms in the cathode biofilms were 35.72%,46.90%,40.17%,35.63%and 21.38%in turns.The abundances of aerobic denitrifying bacteria were 35.72%,52.60%,49.59%,45.08%and 21.38%,respectively.It is speculated that the heterotrophic nitrification-aerobic denitrification was the main pathway for nitrogen removal.

关 键 词：单室无膜MFC 高盐废水 碳氮比 同步硝化反硝化 微生物群落分析 

分 类 号：X703[环境科学与工程—环境工程]