颗粒物对滤膜浓缩/密度梯度分离荧光抗体法检测贾第鞭毛虫和隐孢子虫的影响  

Influence of particles on the detection of Giardia and Cryptosporidium by membrane concentration/density gradient separation and immunofluorescence method

作　　者：张艳芬 李红岩[1] 刘明洋 郑蓓[1] ZHANG Yanfen;LI Hongyan;LIU Mingyang;ZHENG Bei(Key Laboratory of Drinking Water Science and Technology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;School of Environmental and Municipal Engineering,Tianjin Chengjian University,Tianjin 300384,China)

机构地区：[1]中国科学院生态环境研究中心,中国科学院饮用水科学与技术重点实验室,北京100085 [2]天津城建大学环境与市政工程学院,天津300384

出　　处：《环境工程学报》2021年第4期1458-1464,共7页Chinese Journal of Environmental Engineering

基　　金：国家水体污染控制与治理科技重大专项(2018ZX07502-001-012)。

摘　　要：滤膜浓缩/密度梯度分离荧光抗体法因具有成本低廉和性能稳定的特点,目前已列入《城镇供水水质标准检验方法》(CJ/T 141-2018)用于水源水和城镇供水中贾第鞭毛虫和隐孢子虫(简称两虫)的检测。针对实际生产中,水中颗粒物会影响该方法回收率和稳定性的情况,在前期研究的基础上,结合颗粒物大小,进一步探索了浑浊度对滤膜浓缩/密度梯度分离荧光抗体法检测水中两虫的影响。结果表明,颗粒物大小仅对隐孢子虫回收率有影响,对贾第鞭毛虫无显著影响。其中,隐孢子虫在5μm为主要颗粒物的水中获得最高回收率。浑浊度是影响两虫回收率的主要因素,20 NTU以下浑浊度水样适合采用滤膜浓缩法富集浓缩其中的两虫,尤其是3~5 NTU;20 NTU及其以上浑浊度的水样可采用沉淀浓缩法,在30~50 NTU时有最佳回收率。Due to its low cost and stable performance, the membrane concentration/density gradient separation and immunofluorescence method has been listed in the Standard of Water Quality Examination Methods for Urban Water Supply(CJ/T 141-2018) for the detection of Giardia and Cryptosporidium in urban supplied water and source water. In view of the fact that particles in water will affect the recovery and stability of this method,based on the previous research and in combination of particle size, the influence of turbidity and particle size on the detection of Giardia and Cryptosporidium in water was further explored. Results showed that particle size contributing to the turbidity of water only affected the recovery of Cryptosporidium, while had no significant effect on the test of Giardia, of which the highest recovery of Cryptosporidium occurred in water mainly containing particles with 5 μm size. Turbidity is the main factor affecting the recovery of this method. The membrane concentration method was preferred to monitor Giardia and Cryptosporidium in water samples with turbidity below 20 NTU, with the best recovery at 3~5 NTU. The sedimentation concentration method was suitable for water samples with turbidity higher than 20 NTU, with the best recovery for Giardia and Cryptosporidium at 30~50 NTU.

关 键 词：贾第鞭毛虫 隐孢子虫 浑浊度 颗粒物直径 密度梯度分离荧光抗体法 

分 类 号：X832[环境科学与工程—环境工程]