巨噬细胞诱导的C型凝集素在人外周血细胞中的表达及其临床意义  

Expression of Mincle in human peripheral blood cells and its clinical significance

作　　者：李淑萍[1] 陈韵[1] 崔天娇 王晓华[1] 李金红 郑智华[1] LI Shu-ping;CHEN Yun;CUI Tian-jiao;WANG Xiao-hua;LI Jin-hong;ZHENG Zhi-hua(Department of Nephrology,The Seventh Hospital Affiliated to Zhongshan University,Shenzhen 518107,Guangdong Province,China)

机构地区：[1]中山大学附属第七医院肾内科,广东深圳518107

出　　处：《中国生物制品学杂志》2021年第4期428-431,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金青年项目(82000150)。

摘　　要：目的探讨巨噬细胞诱导的C型凝集素(macrophage inducible C-type lectin,Mincle)在人外周血各细胞中的分布情况,并分析其表达量与临床数据的相关性。方法通过流式细胞术检测荧光抗体标记的人外周血细胞,结合荧光信号和FSC/SSC分析Mincle在各类细胞中的表达情况。应用SPSS 25软件,采用双变量线性相关性分析方法,进行Mincle表达量和临床指标的相关性分析。结果Mincle表达于CD11b阳性的髓系白细胞中,不表达于CD11b阴性的白细胞中,Mincle在单核细胞中表达最高,在粒细胞中其次,在淋巴细胞中无表达。Mincle在单核细胞中的表达量与红细胞沉降率呈负相关。结论Mincle在人外周血细胞中主要表达于单核细胞及粒细胞,且部分单核细胞的表达量最高,但未在淋巴细胞中表达。其单核细胞表达量与临床数据存在一定的相关性。Objective To investigate the distribution of macrophage inducible C-type lection(Mincle) in human peripheral blood cells and analyze the correlation of its expression level to the clinical data. Methods Fluorescent antibodylabeled human peripheral blood cells were determined by flow cytometry,while the expression of Mincle in various peripheral blood cells by fluorescent signal combined with FSC/SSC. The correlation of Mincle expression level to clinical indexes was assessed by bivariate linear correlation analysis using SPSS 25 software. Results Mincle was expressed in CD11b-positive myeloid leukocytes but not in CD11b-negative ones. The expression level of Mincle was the highest in monocytes,followed by that in granulocytes. However,no Mincle expression was detected in lymphocytes. The expression level of Mincle in monocytes was correlated with the erythrocyte sedimentation rate(ESR). Conclusion Mincle was mainly expressed in monocytes and granulocytes but not in lymphocytes,of which the expression level was the highest in partial monocytes. The Mincle expression level in monocytes showed a certain correlation to the clinical data.

关 键 词：巨噬细胞诱导的C型凝集素 人外周血细胞 流式细胞术 临床意义 

分 类 号：R392.9[医药卫生—免疫学]