Arab/c/opste N^(6)-methyladenosine reader CPSF30-L recognizes FUE signals to control polyadenylation site choice in liquid-like nuclear bodies  

作　　者：Peizhe Song Junbo Yang Chunling Wang Qiang Lu Linqing Shi Subiding Tayier Guifang Jia 

机构地区：[1]Synthetic and Functional Biomolecules Center,Beijing National Laboratory for Molecular Sciences,Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China [2]Medical Isotopes Research Center and,Department of Radiation Medicine,School of Basic Medical Sciences,Peking University Health Science Center,Beijing 100191,China [3]These authors contributed equally to this article.

出　　处：《Molecular Plant》2021年第4期571-587,共17页分子植物（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(nos.21822702,21820102008,92053109,and 21432002);the National Basic Research Program of China(2017YFA0505201 and 2019YFA0802201).

摘　　要：The biological functions of the epitranscriptomic modification N^(6)-methyladenosine(m^(6)A)in plants are not fully understood.CPSF30-L is a predominant isoform of the polyadenylation factor CPSF30 and consists of CPSF30-S and an m^(6)A-binding YTH domain.Little is known about the biological roles of CPSF30-L and the molecular mechanism underlying its m^(6)A-binding function in alternative polyadenylation.Here,we charac-terized CPSF30-L as an Arabidopsis m^(6)A reader whose m^(6)A-binding function is required for the floral tran-sition and abscisic acid(ABA)response.We found that the m^(6)A-binding activity of CPSF30-L enhances the formation of liquid-like nuclear bodies,where CPSF30-L mainly recognizes m*A-modified far-upstream elements to control polyadenylation site choice.Deficiency of CPSF30-L lengthens the 3'untranslated region of three phenotypes-related transcripts,thereby accelerating their mRNA degradation and leading to late flowering and ABA hypersensitivity.Collectively,this study uncovers a new molecular mechanism for m^(6)A-driven phase separation and polyadenylation in plants.

关 键 词：epitranscriptomics N^(6)-methyladenosine(m^(6)A) m^(6)A reader CPSF30-L phase separation alternative polyadenylation(APA) 

分 类 号：Q94[生物学—植物学]