CPSF30-L-mediated recognition of mRNA m^(6)A modification controls alternative polyadenylation of nitrate signaling-related gene transcripts in Arabidopsis  

作　　者：Yiteng Hou Jing Sun Baixing Wu Yangyang Gao Hongbo Nie Zhentian Nie Shuxuan Quan Yong Wang Xiaofeng Cao Sisi Li 

机构地区：[1]State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [2]Depatment of Biology,Southerm University of Science and Technology.Shenzhen,Guangdong 518055,China [3]State Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agicultural University,Tar'an,Shandong 271018,China [4]University of Chinese Academy of Sciences,Beijing 100049,China [5]Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Being 100101,China [6]Present address:Guangdong Provinclal Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation,Guangdong-Hong Kong Joint Laboratory for RNA Medicine,RNA Biomedical Institute,Medical Research Center,Sun Yat Sen Memorial Hospital,Sun Yat-Sen University,Guangzhou 510120,China [7]These authors contributed equaly to this article.

出　　处：《Molecular Plant》2021年第4期688-699,共12页分子植物（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(31788103 to X.C.,31670247 to Y.W.,31870755 to S.L.,31801063 to Y.H.,31701096 to J.S.,31900435 to B.W.);the Chinese Academy of Sciences(Strategic Priority Research Program XDB27030201 and QYZDY-SSW-SMC022 to X.C.);the Guangdong Innovation Research Team Fund(2016ZT06S172 to S.L.);the Shenzhen Sci-Tech Fund(No.KYTDPT20181011104005 to S.L);the China Postdoctoral Science Foundation(2016M600143 to Y.H.);the Guangdong Science and Technology Department(2020B1212060018 and 2020B1212030004 to B.W.).

摘　　要：N6-methyladenosine(m^(6)A),a ubiquitous internal modification of eukaryotic mRNAs,plays a vital role in almost every aspect of mRNA metabolism.However,there is little evidence documenting the role of m^(6)A in regulating alternative polyadenylation(APA)in plants.APA is controlled by a large protein-RNA complex with many components,including CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30(CPSF30).In Arabidopsis,CPSF30 has two isoforms and the longer isoform(CPSF30-L)contains a YT512-B Homology(YTH)domain,which is unique to plants.In this study,we showed that CPSF30-L YTH domain binds to m^(6)A in v itro.In the cpsf30-2 mutant,the transcripts of many genes including several important nitrate signaling-related genes had shifts in polyadenylation sites that were correlated with m^(6)A peaks,indicating that these gene transcripts carrying m^(6)A tend to be regulated by APA.Wild-type CPSF30-L could rescue the defects in APA and nitrate metabolism in cpsf30-2,but m^(6)A-binding-defective mutants of CPSF30-L could not.Taken together,our results demonstrated that m^(6)A modification regulates APA in Arabidops is and revealed that the m^(6)A reader CPSF30-L affects nitrate signaling by controlling APA,shedding new light on the roles of the m^(6)A modification during RNA 3-end processing in nitrate metabolism.

关 键 词：m^(6)A modification alternative polyadenylation nitrate metabolism CPSF30 

分 类 号：Q943.2[生物学—植物学]