CD248抗体IgG78抑制HFL-1人胚肺成纤维细胞的增殖、迁移  

CD248 specific antibody IgG78 inhibits proliferation,migration of HFL-1 human fetal lung fibroblasts

作　　者：周祥 杨发 韩东晖 许超 刘少杰 鲁诗琪 席文锦 郭张燕 杨安钢 张瑞 秦卫军 温伟红 符兆英[1] ZHOU Xiang;YANG Fa;HAN Donghui;XU Chao;LIU Shaojie;LU Shiqi;XI Wenjin;GUO Zhangyan;YANG Angang;ZHANG Rui;QIN Weijun;WEN Weihong;FU Zhaoying(Department of Immunology,Medical College of Yan'an University,Yan'an,716000;Department of Immunology,School of Basic Medical Sciences,Air Force Medical University,Xi'an 710032;Department of Urology,Xijing Hospital,Air Force Medical University,Xi'an 710032;Medical Research Institute of Northwestern Polytechnical University,Xi'an 710072,China)

机构地区：[1]延安大学医学院免疫学教研室,陕西延安716000 [2]空军军医大学基础医学院免疫学教研室,陕西西安710032 [3]空军军医大学西京医院泌尿外科,陕西西安710032 [4]西北工业大学医学研究院,陕西西安710072

出　　处：《细胞与分子免疫学杂志》2021年第4期289-294,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81772734);陕西创新能力支撑项目(2020PT-021)。

摘　　要：目的研究CD248的全人源单克隆抗体IgG78对HFL-1人胚肺成纤维细胞生物学功能的影响。方法表达和纯化CD248人源性抗体IgG78,用IgG78处理HFL-1细胞后,利用CCK-8实验检测细胞增殖,Transwell^(TM)实验检测细胞迁移,流式细胞术检测细胞凋亡和细胞周期,Western blot法检测CD248及Ⅰ型胶原蛋白α1(COL1A1)和α平滑肌肌动蛋白(α-SMA)的表达变化。结果IgG78可有效抑制HFL-1细胞的增殖和迁移,诱导细胞凋亡,并引起细胞周期的G1期阻滞。IgG78还可抑制HFL-1细胞中CD248及纤维化相关蛋白COL1A1和α-SMA的表达。结论CD248抗体IgG78可抑制成纤维细胞的生物学活性及纤维化相关蛋白的表达,该抗体对CD248特异性增高的肿瘤或纤维化疾病的治疗有潜在应用价值。Objective To investigate the effect of CD248 specific antibody IgG78 on the biological function of HFL-1 human fetal lung fibroblasts.Methods HFL-1 cells were treated with human antibody IgG78 expressed and purified.Cell proliferation was detected by CCK-8 assay,cell migration was detected by Transwell^(TM) assay,cell apoptosis and cell cycle were detected by flow cytometry,and the expressions of CD248,collagen typeⅠalpha 1(COL1A1)and α-smooth muscle actin(α-SMA)were detected by Western blotting.Results IgG78 effectively inhibited the proliferation and migration of HFL-1 cells,induced apoptosis,and arrested cell cycle at G1 phase.IgG78 also inhibited the expression of CD248 and fibrosis-related proteins COL1A1 and α-SMA in HFL-1 cells.Conclusion CD248 specific antibody IgG78 can inhibit the biological activity of fibroblasts and the expression of fibrosis-related proteins,indicating that it can be used in the treatment of tumors and fibrotic diseases with specific increase in CD248 expression.

关 键 词：成纤维细胞 CD248 抗体 肿瘤 纤维化疾病 

分 类 号：S853.53[农业科学—临床兽医学] R392.12[农业科学—兽医学]