温州市2019年新诊断HIV-1感染者的病毒基因型分析  

Genotype Analysis of Newly Diagnosed HIV-1 Infected Cases in Wenzhou in 2019

作　　者：朱传新[1] 郑文力[1] 金聪囡 吴矛矛 孙宝昌[1] 吴可可[1] 钭慧芬[1] ZHU Chuanxin;ZHENG Wenli;JIN Congnan;WU Maomao;SUN Baochang;WU Keke;TOU Huifen(Wenzhou Center for Disease Control and Prevention,Wenzhou 325000,Zhejiang,China;School of Laboratory Medicine and Life Science,Wenzhou Medical University 325000,Zhejiang)

机构地区：[1]温州市疾病预防控制中心,浙江温州325000 [2]温州医科大学检验医学院(生命科学学院),浙江温州325000

出　　处：《中国艾滋病性病》2021年第4期337-342,共6页Chinese Journal of Aids & STD

基　　金：温州市科技局项目(Y2020554,Y2020542)。

摘　　要：目的分析温州市2019年新诊断HIV-1感染者的病毒基因型,揭示亚型分布特点及流行趋势。方法采集232例HIV-1感染者血浆样本,采用巢式PCR扩增pol区基因,于Los Alamos HIV database下载参考序列,构建系统进化树确定基因亚型,利用HIV Blast工具进行同源性分析。结果获得199例感染者的pol区基因序列,以异性性传播(61.3%,122/199)为主,其次是同性性传播(23.1%,46/199)。同性性传播感染者以19~49岁(78.3%,36/46)的青壮年为主,多为无配偶。共检出8种基因亚型,以CRF07_BC(47.2%,94/199)和CRF01_AE(29.1%,58/199)为主;其次是CRF08_BC(6.5%,13/199)、C(6.0%,12/199)、B(4.5%,9/199)、CRF85_BC(2.5%,5/199)、CRF55_01B(1.5%,3/199)和CRF59_01B(0.5%,1/199)。检出2种独特重组型(URFs),分别是URF(CRF01_AE/BC)(1.0%,2/199)和URF(B/C)(1.0%,2/199)。发现20个传播簇,总成簇比例为20.6%(41/199),CRF07_BC、CRF01_AE和CRF08_BC的传播簇分别为6个、8个和2个;C、CRF55_01B、B和URF(CRF01_AE/BC)的传播簇均为1个。结论温州市2019年新诊断HIV-1感染者病毒基因亚型多样,以CRF07_BC和CRF01_AE为主,C亚型占比偏高,成簇率约20%。应加强对HIV-1亚型和传播簇的监测,掌握动态变化趋势。Objective To analyze the virus genotypes of newly diagnosed HIV-1 infected cases and reveal the distribution of subtypes and epidemic trends in Wenzhou, 2019. Methods Plasma samples were collected from 232 HIV-1 infected patients. Pol gene regions were amplified by nested PCR. Reference sequences were downloaded from Los Alamos HIV Database. Phylogenetic trees were constructed to determine gene subtypes, and the HIV Blast tool was used for homology analysis. Results Heterosexual transmission dominated in the study subjects(61.3%, 122/199), followed by homosexual transmission(23.1%, 46/199). The majority of subjects infected by homosexual transmission were young adults aged 19-49 years(78.3%, 36/46), and most of whom without a spouse. There were 8 subtypes, mainly CRF07_BC(47.2%, 94/199) and CRF01_AE(29.1%, 58/199), followed by CRF08_BC(6.5%, 13/199), C(6.0%, 12/199), B(4.5%, 9/199), CRF85_BC(2.5%, 5/199).CRF55_01B(1.5%, 3/199), and CRF59_01B(0.5%, 1/199), CRF85_BC(2.5%, 5/199).Two URFs were found, including URF(CRF01_AE/BC)(1.0%, 2/199) and URF(BC)(1.0%, 2/199). Twenty transmission clusters were found, with the total clustered rate as 20.6%(41/199), including 6 CRF07_BC transmission clusters, 8 CRF01_AE transmission clusters, and 2 CRF08_BC transmission clusters. Each C, CRF55_01B, B, and URF(CRF01_AE/BC) transmission cluster was identified. Conclusion The subtypes of HIV infected by newly diagnosed Wenzhou cases in 2019 were diverse. CRF07_BC and CRF01_AE appeared as the main subtypes. Subtype C accounted for a relatively higher proportion, with the clustered rate as almost 20%. Programs regarding the trends of subtypes changes and early warning of HIV-1 subtypes and transmission clusters should be set up and dynamically under surveillance.

关 键 词：1型艾滋病病毒 基因型 传播簇 同源性 

分 类 号：R373.9[医药卫生—病原生物学]