普通小麦Soru#1×Naxos群体代谢物的QTL定位  

Mapping the QTLs of metabolites in RIL population of common wheat Soru#1×Naxos

作　　者：薛明云 陈杰 陈伟[2] 吴纪中[3] 兰彩霞[1] 李东芹 XUE Mingyun;CHEN Jie;CHEN Wei;WU Jizhong;LAN Caixia;LI Dongqin(College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Key Laboratory of Crop Genetics and Improvement,Wuhan 430070,China;Institute of Germplasm Resources and Biotechnology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]华中农业大学植物科学技术学院,武汉430070 [2]华中农业大学作物遗传改良国家重点实验室,武汉430070 [3]江苏省农业科学院种质资源与生物技术研究所,南京210014

出　　处：《华中农业大学学报》2021年第3期126-132,共7页Journal of Huazhong Agricultural University

基　　金：中央高校基本科研业务费专项(2662019PY008);国家自然科学基金青年科学基金项目(32001541)。

摘　　要：以Soru#1×Naxos构建的RIL群体为材料,对成熟籽粒进行代谢组学测定。结果显示:所检测到的478种已知代谢物含量具有较高遗传力和不同程度的含量变异,这些代谢物中处于同一代谢通路的结构相似的物质具有相似的含量分布规律。对这些代谢物含量数据进行QTL定位,共获得236种代谢物对应的385个mQTL位点,其中5个位点为被5种以上代谢物共定位的热点区域,分别位于5A(2个热点区域)、5B(1个)、6A(1个)和6B(1个)染色体。这些热点中有3个由Naxos提供,2个由Soru#1提供。The contents of metabolites in the mature kernels of each line from the RIL population constructed by crossing the common wheat Soru#1 with Naxos were determined.The results showed that the contents of 478 known metabolites detected had high broad-sense heritability and varying degrees of content variation.Among these metabolites,the structurally similar metabolite involved in the same metabolic pathway had the similar rule of content distribution.The QTL mapping of contents of metabolites detected resulted in a total of 385 mQTL loci corresponding to 236 metabolites.Five of them are hotspots co-mapped by more than five metabolites,located on the chromosome 5A with 2 hotspots,5B with 1 hotspot,6A with 1 hotspot,and 6B with 1 hotspot,respectively.Three of these hotspots are from Naxos and two are from Soru#1.

关 键 词：小麦 代谢组学 mRIL群体 QTL定位 籽粒代谢谱 候选基因鉴定 

分 类 号：S512.1[农业科学—作物学]