不同中效稀释粉对猪精液常温保存效果的比较  

Comparison of effects of different medium-effect dilution powders on boar semen preservation at normal temperature

作　　者：赵展展 柳梦圆 陈雷 雷明刚[1] ZHAO Zhanzhan;LIU Mengyuan;CHEN Lei;LEI Minggang(Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction( Huazhong Agricultural University ),Ministry of Education,Wuhan 430070,China;Hubei Jinxu Boar Breeding Station Co.,Ltd,Xiangyang 441100,China)

机构地区：[1]华中农业大学农业动物遗传育种与繁殖教育部重点实验室,武汉430070 [2]湖北金旭种公猪站有限公司,襄阳441100

出　　处：《华中农业大学学报》2021年第3期212-220,共9页Journal of Huazhong Agricultural University

基　　金：国家生猪产业技术体系建设专项(CARS-36)。

摘　　要：为对比不同中效稀释粉对猪精液常温保存效果的影响,试验选取业内知名度较高的9种中效稀释粉,其中国内3种和国外6种,分别编号为A、B、C、D、E、F、G、H和I,用12头杜洛克公猪精液进行了常温保存试验;对活力有效期内精液的pH值、活力、有效保存时长、畸形率以及顶体和质膜完整率的变化情况进行统计分析。结果显示:稀释粉E的活力在保存3 d后显著高于稀释粉C、D、F、G(P<0.05);稀释粉E、I的有效保存时长最长,达10.91 d和10.33 d,显著高于另外6种(P<0.05);稀释粉D、E、G、H稀释的精液畸形率较低,其中稀释粉D、E显著低于另外4种(P<0.05);稀释粉A稀释的精液自第2天后精子顶体完整率一直最高,显著高于稀释粉C、D、F(P<0.05);稀释粉E、H的质膜完整率较高,其中稀释粉H显著高于稀释粉D、F(P<0.05)。综上,9种中效稀释粉常温保存的猪精液质量达国标和产品标准的合格率均为88.89%,其中稀释粉E、H、I效果最好;稀释粉对精液质量影响的评定宜囊括多项指标进行综合考量。To compare the effects of different medium-effect dilution powders on the quality of boar semen stored at normal temperature,9 kinds of medium-effect dilution powders were selected,including 3 kinds of domestic and 6 kinds of foreign products,numbered as A,B,C,D,E,F,G,H and I,respectively.Boar semen from 12 Duroc was used for normal temperature preservation by measuring the pH value,motility,effective preservation time,deformity rate,acrosome integrity rate and plasma membrane integrity rate during the effective period of sperm motility.The results showed that the sperm motility of diluted powder E was significantly higher than that of diluted powder C,D,F and G after storage for 3 days(P<0.05).The semen diluted with diluted powder E and I had the longest effective storage time,reaching 10.91 d and 10.33 d,significantly higher than the other 6(P<0.05).The deformities of diluted powder D,E,G and H were lower,and the deformities of diluted powder D and E were significantly lower than the other 4(P<0.05).The sperm acrosome integrity rate of diluted powder A was always the highest after the 2nd day,which was significantly higher than that of diluted powder C,D and F(P<0.05).The sperm membrane integrity rate of diluted powder E and H was significantly higher than that of diluted powder D and F(P<0.05).Taken together,when boar semen diluted with 9 kinds of medium-effect dilution powders was stored at room temperature,the qualified rate reached 88.89%of the national standard and product standard.Among them,dilution powder E,H and I have the best effect.The evaluation of diluent powder’s influence on boar semen quality should include several indexes.

关 键 词：猪精液 常温保存 中效稀释粉 有效保存 精液质量 人工授精 

分 类 号：S814.8[农业科学—畜牧学] S828.8[农业科学—畜牧兽医]