HPLC梯度洗脱法同时测定慢肝解郁胶囊中8种成分的含量  

Simultaneous Determination of Eight Constituents in Mangan Jieyu Capsules by HPLC Gradient Elution Method

作　　者：沈晓庆[1] 韦杏[2] SHEN Xiao-qing;WEI Xing(Department of Pharmacy Management,Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang ChinaGuangxi University of Chinese Medicine,Nanning China)

机构地区：[1]辽宁中医药大学附属医院药学管理部,辽宁沈阳110032 [2]广西中医药大学,广西南宁530001

出　　处：《中国现代中药》2021年第4期709-713,733,共6页Modern Chinese Medicine

基　　金：广西重点实验室建设项目(17-259-34)。

摘　　要：目的:建立高效液相色谱法(HPLC)梯度洗脱法同时测定慢肝解郁胶囊中柴胡皂苷a、柴胡皂苷d、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA、延胡索乙素、去氢紫堇碱和紫堇碱的含量。方法:采用Waters sunfire C18色谱柱(250 mm×4.6 mm,5μm),柱温:30℃;流动相:乙腈-0.1%冰乙酸溶液,梯度洗脱;检测波长:210 nm(检测柴胡皂苷a和柴胡皂苷d)和280 nm(检测隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA、延胡索乙素、去氢紫堇碱和紫堇碱);体积流量:1.0 mL·min-1。结果:柴胡皂苷a、柴胡皂苷d、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA、延胡索乙素、去氢紫堇碱和紫堇碱分别在3.41~68.20(r=0.9999)、2.58~51.60(r=0.9992)、0.99~19.80(r=0.9993)、0.77~15.40(r=0.9997)、1.98~39.60(r=0.9995)、3.17~63.40(r=0.9994)、2.89~57.80(r=0.9992)、1.81~36.20μg·mL-1(r=0.9996)线性关系良好,平均加样回收率及RSD分别为99.90%(RSD=0.93%)、98.81%(RSD=1.02%)、96.99%(RSD=1.27%)、97.48%(RSD=1.15%)、98.78%(RSD=0.83%)、100.01%(RSD=0.78%)、99.06%(RSD=1.12%)、97.92%(RSD=1.30%)。结论:该方法操作简便、重复性好,可用于慢肝解郁胶囊的质量控制。Objective:To establish a HPLC gradient elution method for simultaneous determination of saikosaponin a,saikosaponin d,cryptotanshinone,tanshinoneⅠ,tanshinoneⅡA,tetrahydropalmatine,dehydrocavidine and corydaline in Mangan Jieyu capsules.Methods:HPLC analysis was performed on an Waters sunfire C18(250 mm×4.6 mm,5μm)chromatographic column with gradient elution using acetonitrile-0.1%acetic acid solution as mobile phase at the flow rate of 1.0 mL·min-1 and the column temperature was 30℃.The detection wavelength were set at 210 nm for saikosaponin a and saikosaponin d,and 280 nm for cryptotanshinone,tanshinoneⅠ,tanshinoneⅡA,tetrahydropalmatine,dehydrocavidine and corydaline.Results:Saikosaponin a,saikosaponin d,cryptotanshinone,tanshinoneⅠ,tanshinoneⅡA,tetrahydropalmatine,dehydrocavidine and corydaline showed good linear relationships within the ranges of 3.41-68.20(r=0.9999),2.58-51.60(r=0.9992),0.99-19.80(r=0.9993),0.77-15.40(r=0.9997),1.98-39.60(r=0.9995),3.17-63.40(r=0.9994),2.89-57.80(r=0.9992),1.81-36.20μg·mL-1(r=0.9996).The average recoveries were 99.90%,98.81%,96.99%,97.48%,98.78%,100.01%,99.06%,97.92%with the RSDs of 0.93%,1.02%,1.27%,1.15%,0.83%,0.78%,1.12%and 1.30%,respectively.Conclusion:The method is easy to operate and repeatable,can be used for the quality control of Mangan Jieyu capsules.

关 键 词：慢肝解郁胶囊 含量测定 高效液相色谱法 梯度洗脱法 

分 类 号：R286[医药卫生—中药学]