多重耐药ST17无乳链球菌体外抗菌活性中药筛选  

Screening of Chinese Herbal Medicines with Antibacterial Activity against Multi-Drug Resistant ST17 Streptococcus agalactiae in Vitro

作　　者：程招敏[1] 潘敏旋 柏彩英[2] 李亮 李炜鑫 周强[1] 柯培锋[1] CHENG Zhao-Min;PAN Min-Xuan;BO Cai-Ying;LI Liang;LI Wei-Xin;ZHOU Qiang;KE Pei-Feng(Dept.of Clinical Laboratory Medicine,Guangdong Provincial Hospital of Chinese Medicine,Guangzhou 510120 Guangdong,China;Dept.of Clinical Laboratory Medicine,Guangdong Provincial Maternal and Child Health Institu te,Guangzhou 510010 Guangdong,China;Faculty of Chinese Medicine Science,Guangxi University of Chinese Medicine,Nanning 530200 Guangxi,China)

机构地区：[1]广东省中医院检验科,广东广州510120 [2]广东省妇幼保健院检验科,广东广州510010 [3]广西中医药大学赛恩斯新医药学院,广西南宁530200

出　　处：《广州中医药大学学报》2021年第6期1217-1222,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药局科研基金资助项目(编号:20181102)。

摘　　要：【目的】筛选对多重耐药ST17无乳链球菌有抑菌活性的中药,为其引起感染的控制提供新的途径。【方法】从74种中药配方颗粒中,筛选对多重耐药ST17菌株有抑菌活性的中药。检测有中等以上抑菌活性中药最小抑菌浓度(MIC)、最小杀菌浓度(MBC)以及分别与红霉素或克林霉素的联合抑菌效果。【结果】共筛选出黄柏、黄连、桑白皮、甘草、五味子、大黄、鸡血藤和地榆等8种有中等以上抑菌活性的中药,其MIC依次为2、0.512、1、16、32、64、8、8、8 mg·mL-1,MBC依次为8、1、8、32、64、32、16~32、16~32 mg·mL-1。与黄连联合用药时,所有红霉素或克林霉素耐药株FIC≤0.5。【结论】黄柏、黄连、桑白皮、甘草、五味子、大黄、鸡血藤和地榆对多重耐药ST17无乳链球菌有良好的体外抑菌活性。对于红霉素或克林霉素耐药株,与黄连联合用药起协同作用。Objective To screen Chinese herbal medicines with antibacterial activity against multi-drug resistant Streptococcus agalactiae ST17 strains,so as to provide a new way to control the infection. Methods From 74 kinds of Chinese herbal medicine granules,the Chinese herbal medicines with antibacterial activity against multi-drug resistant Streptococcus agalactiae ST17 strains were screened. The minimum inhibitory concentration(MIC),minimum bactericidal concentration(MBC) of Chinese herbal medicines with above moderate antibacterial activity,and the bacteriostatic effects in combination with erythromycin or clindamycin were measured. Results A total of 8 Chinese herbal medicines with above moderate antibacterial activity were selected out, including Phellodendri Chinensis Cortex,Coptidis Rhizoma,Mori Cortex,Glycyrrhizae Radix et Rhizoma,Schisandrae Chinensis Fructus,Rhei Radix et Rhizoma,Spatholobi Caulis and Sanguisorbae Radix,the corresponding MIC was 2,0.512,1,16,32,64,8,8,8 mg·mL-1,and MBC was 8,1,8,32,64,32,16-32,16-32 mg·mL-1,respectively. The fractional inhibitory concentration(FIC) of erythromycin or clindamycin against resistant strains in combination with Coptidis Rhizoma was less than or equal to 0.5. Conclusion Phellodendri Chinensis Cortex, Coptidis Rhizoma, Mori Cortex, Glycyrrhizae Radix et Rhizoma, Schisandrae Chinensis Fructus, Rhei Radix et Rhizoma, Spatholobi Caulis and Sanguisorbae Radix have good antibacterial activity against multidrug-resistant Streptococcus agalactiae ST17 strains in vitro. Erythromycin or clindamycin associated with Coptidis Rhizoma has a synergistic effect against resistant strains.

关 键 词：多重耐药 无乳链球菌 中药颗粒剂 最小抑菌浓度 最小杀菌浓度 

分 类 号：R282.5[医药卫生—中药学]