多肋藻雌配子体种质保存和扩培的温光条件研究  

Screening Temperature and Illumination Conditions for the Germplasm Preservation and Efficient Propagation of Female Gametophyte of Costaria Costata(C.Agardh)Saunder

作　　者：赛珊 赵楠 罗世菊 王伟伟 武瑞娜 李晓捷 SAI Shan;ZHAO Nan;LUO Shi-ju;WANG Wei-wei;WU Rui-na;LI Xiao-jie(Shandong Oriental Ocean Sci-tech Co.Ltd.,National Algae and Sea Cucumber Engineering and Technique Research Center,Shandong Algae and Sea Cucumber Technology Innovation Center,Shandong Key Laboratory of Genetic Improvement&Efficient Culture of Marine Alage,Yantai,Shandong 264003,China)

机构地区：[1]山东东方海洋科技股份有限公司,国家海藻与海参工程技术研究中心,山东省海藻与海参技术创新中心,山东省海藻遗传育种与栽培技术重点实验室,山东烟台264003

出　　处：《水产养殖》2021年第5期24-28,共5页Journal of Aquaculture

基　　金：国家重点研发计划“蓝色粮仓科技创新”重点专项(2018YFD0901500);现代农业产业技术体系专项资金(CARS-50);烟台市科技计划项目(2018LJRC067);山东省重点实验室专项建设计划(SDKL2017009);山东省现代农业产业技术体系建设专项资金(SDAIT-26-11)。

摘　　要：跟踪观察了温度和光照强度双因子对多肋藻雌配子体生长的影响,旨在探明其适宜的种质保存与扩增培养的温光条件。试验设置3个温度,分别为10、15和20℃,每个温度下设5个光照强度,分别为10、20、40、60和80μmol/(m^(2)·s),实验进行了4个月。结果表明,配子体在10℃,光强≤10μmol/(m^(2)·s)时适宜种质保存,15~20℃、20μmol/(m^(2)·s)时适宜短期扩增培养,15℃、20μmol/(m^(2)·s)时适宜长期扩增培养。The growth of female gametophytes of Costaria costata were tested under dual factors of temperature and illumination,aiming to screen the suitable conditions for germplasm preservation and efficient propagation.The experiment set three temperatures at 10℃,15℃and 20℃,set five illuminations at each temperature,namely 10μmol/(m^(2)·s),20μmol/(m^(2)·s),40μmol/(m^(2)·s),60μmol/(m^(2)·s),80μmol/(m^(2)·s),and lasted four months.The results indicated that the optimum condition for the germplasm preservation of female gametophyte was 10℃and 10μmol/(m^(2)·s)or less,for short-term culture Was 15~20℃and 20μmol/(m^(2)·s),and for long-term culture was 15℃and 20μmol/(m^(2)·s).

关 键 词：多肋藻雌配子体 温度 光照强度 种质保存 扩增培养 

分 类 号：S968.4[农业科学—水产养殖]