正反杂交对两种生态型少花龙葵F_(1)代生长及硒积累的影响  

Effects of Reciprocal Crosses on the Growth and Selenium Accumulation in F_(1) Generation of Two Ecotypes of Solanum photeinocarpum

作　　者：黄科文[1] 曹艺兰 郑阳霞[1] 李良 刘继[3] 林立金[4] 廖明安[1] HUANG Kewen;CAO Yilan;ZHENG Yangxia;LI Liang;LIU Ji;LIN Lijin;LIAO Ming'an(College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China;Agricultural Technology Soil and Fertilizer Station of Ganzi Tibetan Autonomous Prefecture,Kangding 626000,China;Chengdu Academy of Agriculture and Forestry Sciences,Chengdu 611130,China;Institute of Pomology and Olericulture,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学园艺学院,四川成都611130 [2]甘孜藏族自治州农技土肥站,四川康定626000 [3]成都市农林科学院,四川成都611130 [4]四川农业大学果蔬研究所,四川成都611130

出　　处：《云南农业大学学报（自然科学版）》2021年第3期377-383,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：四川省教育厅项目(17ZB0342)。

摘　　要：【目的】研究正反杂交对少花龙葵(Solanum photeinocarpum)F_(1)代生理特性及富硒能力的影响,为富硒少花龙葵的杂交选育提供参考。【方法】以农田生态型和矿山生态型少花龙葵为亲本,通过硒含量为10 mg/kg的土壤盆栽试验,研究正反杂交对少花龙葵F_(1)代的生物量、光合色素含量、抗氧化酶活性以及硒积累量的影响。【结果】与自交F_(1)代相比,正反杂交显著提高了少花龙葵F_(1)代叶片的SOD活性、CAT活性以及可溶性蛋白含量(P<0.05)。2种生态型少花龙葵的正反杂交F_(1)代的根系和地上部分的生物量、硒积累量均显著高于其自交F_(1)代(P<0.05),并且以农田生态型少花龙葵为母本、矿山生态型少花龙葵为父本的杂交后代的生长和富硒能力更强。【结论】以农田生态型和矿山生态型少花龙葵为亲本杂交,可使其F_(1)代的生长、抗逆性以及硒积累等性状表现出超亲优势。[Purpose]We investigated the effects of reciprocal crosses on the physiological characteristics and selenium-enriched ability of F1 generations in Solanum photeinocarpum to provide reference for the cross breeding of selenium-enriched S.photeinocarpum.[Method]Farmland and mining ecotypes of S.photeinocarpum were cultivated in pot experiment with 10 mg/kg selenium,the effects of reciprocal crosses on the biomass,photosynthetic pigment content,antioxidant enzyme activity and selenium accumulation of S.photeinocarpum F1 generation were studied.[Result]Compared with the self-bred F1 generations,reciprocal crosses significantly increased the SOD activity,CAT activity,soluble protein content in leaves of S.photeinocarpum F1 generations(P<0.05).The biomass and selenium accumulation in roots and shoots of F1 generations of two ecotypes of S.photeinocarpum were significantly higher than that of their self-bred F1 generations(P<0.05),and the growth and selenium-enriched ability of hybrid progenies with farmland ecotype S.photeinocarpum was used as female parent and mining ecotype S.photeinocarpum was used as male parent were stronger.[Conclusion]The traits of growth,stress resistance,and selenium accumulation of F1 generations of farmland and mining ecotypes of S.photeinocarpum were superior to those of their parents.

关 键 词：少花龙葵 正反杂交 生态型 硒 

分 类 号：Q949.777.703[生物学—植物学]