补肾益精健脾养血方对贫血大鼠骨髓细胞凋亡的影响  

Effects of Kidney-Nourishing,Marrow-Replenishing,Spleen-Invigorating,Blood-Nourishing Decoction on Marrow Cells Apoptosis in Rats with Anemia

作　　者：王正引[1] 陈燕[1] 刘蔚雯[1] 郭明章[2] 张小如[1] WANG Zheng-Yin;CHEN Yan;LIU Wei-Wen;GUO Ming-Zhang;ZHANG Xiao-Ru(Pharmacy School,Fujian University of Traditional Chinese Medicine,Fuzhou 350122 Fujian,China;Chinese Medicine School,Fujian University of Traditional Chinese Medicine,Fuzhou 350122 Fujian,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建中医药大学中医学院,福建福州350122

出　　处：《广州中医药大学学报》2021年第5期997-1002,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：福建省自然科学基金资助项目(编号:2017J01834)。

摘　　要：【目的】观察补肾益精健脾养血方对贫血大鼠骨髓细胞凋亡的影响。【方法】将48只Wistar大鼠随机分为空白组,模型组,中药低、中、高剂量组,肌苷组(阳性对照),每组8只。模型组及各给药组以腹腔注射环磷酰胺法建立贫血模型,空白组给予等容积生理盐水腹腔注射。成功造模后,中药低、中、高剂量组给予补肾益精健脾养血方6、12、24 g·kg^(-1)·d^(-1)灌胃,肌苷组给予肌苷0.05 g·kg^(-1)·d^(-1)灌胃,空白组和模型组则给予等容积的生理盐水灌胃,给药2周。全自动血液分析仪检测外周血红细胞数、白细胞数、血小板数及血红蛋白含量。末端脱氧核苷酸转移酶介导的dUTP缺口末端标记(TUNEL)法测定骨髓细胞凋亡百分率,光镜下观察骨髓细胞凋亡情况。蛋白免疫印迹法检测骨髓细胞B-细胞淋巴瘤/白血病-2原癌基因(Bcl-2)、Bcl-2相关X蛋白(Bax)、半胱天冬氨酸蛋白酶(Caspase)3、Caspase 8的表达。【结果】与空白组比较,模型组血红细胞数、白细胞数、血小板数、血红蛋白含量及骨髓细胞Bcl-2蛋白表达水平降低(P <0.05),光镜下骨髓凋亡细胞增多,骨髓细胞凋亡百分率及骨髓细胞Bax、Caspase 3、Caspase 8蛋白表达水平升高(P <0.05)。经补肾益精健脾养血方干预后,上述指标得到明显改善。【结论】补肾益精健脾养血方抗贫血作用机制可能与其减少骨髓细胞凋亡有关。Objective To observe the effects of Kidney-Nourishing,Marrow-Replenishing,Spleen-Invigorating,Blood-Nourishing Decoction on marrow cells apoptosis in rats with anemia.Methods A total of 48 Wistar rats were randomly divided into blank group,model group,low-,middle-,high-dose Chinese medicine groups and carnine group(positive control),8 rats in each group.A anemia model was established in the model group and various medication groups by intraperitoneal injection of cyclophosphamide,and the blank group was given intraperitoneal injection of the same volume of normal saline.After successful modeling,the low-,middle-and high-dose Chinese medicine groups were given intragastric administration of Kidney-Nourishing,MarrowReplenishing,Spleen-Invigorating,Blood-Nourishing Decoction at the dosage of 6,12,24 g·kg^(-1)·d^(-1),respectively,the carnine group was given intragastric administration of carnine at the dosage of 0.05 g·kg^(-1)·d^(-1),and the blank group and model group were given intragastric administration of the same volume of normal saline,the treatment lasting 2 weeks.After medication,the peripheral hemogram was tested by full-automatic blood analyzer,the percent of marrow cells apoptosis was measured by TUNEL assay,and features of marrow cells apoptosis were observed under optical microscope,and the protein expression levels of B-cell lymphoma/leukemia-2(Bcl-2),Bcl-2 associated X Protein(Bax),cysteinyl aspartate-specific protease(Caspase)3 and Caspase 8 in marrow cells were detected by Western blotting assay.Results Compared with the blank group,the counts of erythrocytes,leukocytes,platelets and content of hemoglobin as well as protein expression level of Bcl-2 in marrow cells were decreased in the model group(P<0.05),more marrow apoptotic cells were seen under optical microscope,and the percent of marrow cells apoptosis and protein expression levels of Bax,Caspase 3and Caspase 8 were increased(P<0.05).After intervention of Kidney-Nourishing,Marrow-Replenishing,Spleen-Invigorating,Blood-Nourishing Decoction

关 键 词：补肾益精健脾养血方 贫血 骨髓细胞 细胞凋亡 大鼠 

分 类 号：R285.5[医药卫生—中药学]