新型琥珀酸脱氢酶抑制剂类杀菌剂氟唑菌酰羟胺对水稻恶苗病的防治研究  

Efficacy of a novel succinate dehydrogenase inhibitor pydiflumetofen to control rice bakanae disease caused by Fusarium fujikuroi

作　　者：侯毅平[1] 曲香蒲 蔡小威 武洛宇 陈长军[1] 王建新[1] 田宝华 周明国[1] HOU Yiping;QU Xiangpu;CAI Xiaowei;WU Luoyu;CHEN Changjun;WANG Jianxin;TIAN Baohua;ZHOU Mingguo(College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China;Syngenta(China)Investment Co.,Ltd.,Shanghai 200120,China)

机构地区：[1]南京农业大学植物保护学院,南京210095 [2]先正达(中国)投资有限公司,上海200120

出　　处：《农药学学报》2021年第3期483-491,共9页Chinese Journal of Pesticide Science

基　　金：National Natural Science Foundation of China(31972307);Jiangsu Agriculture Science and Technology Innovation Fund(CX(18)1003);the Fundamental Research Funds for the Central Universities(KYXK202011).

摘　　要：藤仓镰孢菌Fusarium fujikuroi是引起水稻恶苗病的主要致病菌,氟唑菌酰羟胺(pydiflumetofen)是一种新型的琥珀酸脱氢酶抑制剂(SDHI)类杀菌剂。氟唑菌酰羟胺对藤仓镰孢菌的生物活性及其对水稻恶苗病的防治效果尚不明确。本研究从江苏和浙江两省不同地区分离鉴定出100株藤仓镰孢菌,通过菌丝生长速率法和孢子萌发法建立了藤仓镰孢菌对氟唑菌酰羟胺的敏感性基线,测定了该药剂对藤仓镰孢菌的生理影响及其对水稻恶苗病的防治效果。结果表明:氟唑菌酰羟胺对100株藤仓镰孢菌菌丝生长的EC_(50)值的范围为0.0125~0.1118μg/mL,平均值为0.0503(±0.0029)μg/mL;对100株藤仓镰孢菌孢子萌发的EC_(50)值的范围在0.0001~0.0245μg/mL之间,平均值为0.0038(±0.0005)μg/mL。用0.05μg/mL(EC_(50)值)或1.3μg/mL(EC_(90)值)的氟唑菌酰羟胺处理藤仓镰孢菌菌丝体后,菌丝体顶端分支增多、产孢量下降、细胞膜和细胞器(如线粒体)受损、细胞内含物渗漏增加,胞外多糖产量无变化,而过氧化物酶活性下降。用200 g/L氟唑菌酰羟胺悬浮剂按有效成分10、15和20 g的剂量拌种处理100 kg水稻种子,对水稻恶苗病的防治效果分别为94.77%、98.60%和100%。研究结果说明,氟唑菌酰羟胺对藤仓镰孢菌高活性,对水稻恶苗病具有优异的防治效果。Fusarium fujikuroi is the main pathogen causing rice bakanae disease.The fungicide pydiflumetofen is a novel succinate dehydrogenase inhibitor(SDHI).The activity of pydiflumetofen against F.fujikuroi and its efficacy of controlling rice bakanae disease is obscure.In this study,100 strains of F.fujikuroi were isolated from different regions of Jiangsu and Zhejiang Province in China.The baseline sensitivity of F.fujikuroi to pydiflumetofen was established by mycelial growth assay and spore germination assay respectively,and physiological effect of the fungicide against F.fujikuroi and its control efficacy were investagated.The EC_(50)(half effective concentration)values of pydiflumetofen on restraining mycelial growth against F.fujikuroi population(n=100)ranged from 0.0125μg/mL to 0.1118μg/mL and the mean value was 0.0503(±0.0029)μg/mL.The EC_(50) values of pydiflumetofen on inhibiting spore germination distributed from 0.0001μg/mL to 0.0245μg/mL and the mean value was 0.0038(±0.0005)μg/mL.Treated with 0.05μg/mL(EC_(50) value)or 1.3μg/mL(EC_(90) value)pydiflumetofen,the offshoot of top mycelia of F.fujikuroi increased while sporulation production descreased.The membrane structure and organelles(eg.mitochondria)were damaged and the intracellular plasma leakage increased through imperfect membrane.There was no significant difference in the exopolysaccharides production while peroxidase activity declined after treated with pydiflumetofen.Control efficacy of 10,15 and 20 g a.i./100 kg seeds of pydiflumetofen against rice bakanae disease by seed dressing is 94.77%,98.60%and 100%respectively.Above results indicate that pydiflumetofen has very high activity against F.fujikuroi and is a nice fungicide for controlling rice bakanae disease caused by F.fujikuroi.

关 键 词：氟唑菌酰羟胺 水稻恶苗病 藤仓镰孢菌 敏感性基线 防治效果 

分 类 号：S482.2[农业科学—农药学] TQ450.2[农业科学—植物保护]