用于重组蛋白药物生产的CHO细胞无血清培养基的研究进展  

Advances of Serum-Free Medium for CHO Cells for the Production of Recombinant Protein

作　　者：李伟风 樊振林 张洹瑜 林艳 王天云 LI Weifeng;FAN Zhenlin;ZHANG Huanyu;LIN Yan;WANG Tianyun(Department of Biochemistry and Molecular Biology,School of Basic Medicine,Xinxiang Medical University,Xinxiang 453003,China;Henan International Joint Laboratory of Recombinant Therapeutic Protein Expression System,Xinxiang 453000,China;School of Nursing,Xinxiang Medical University,Xinxiang 453003,China)

机构地区：[1]新乡医学院基础医学院生物化学与分子生物学教研室,新乡453003 [2]河南省重组药物蛋白表达系统国际联合实验室,新乡453000 [3]新乡医学院护理学院,新乡453003

出　　处：《中国细胞生物学学报》2021年第4期905-916,共12页Chinese Journal of Cell Biology

基　　金：河南省高等学校重点科研项目计划(批准号:20A350007);河南省高校重点科研项目(批准号:19A350008)资助的课题。

摘　　要：哺乳动物表达系统因其具有类似于人源化细胞的翻译后修饰方式,已经成为重组蛋白药物生产的主要表达系统。中国仓鼠卵巢(Chinese hamster ovary,CHO)细胞是生产重组蛋白的理想哺乳动物细胞宿主,目前近70%批准上市的重组蛋白药物是由CHO细胞生产的。常规细胞培养所用的培养基需要补充血清才能正常生长,但血清存在来源批次不一、下游分离纯化困难、支原体污染潜在风险等缺点,因此,CHO细胞生产重组蛋白药物要求必须用无血清培养基培养避免以上问题产生。近年来,围绕无血清培养基进行了大量研究,并取得了显著进展。该文综述了CHO细胞的特性、无血清培养基的基础成分及作用,以及一些特殊添加剂的作用等方面的研究进展。Mammalian expression system has become the main expression system for the production of recombinant protein,due to its similarity of the post-translational modification to the human cells.CHO (Chinese hamster ovary) cells are ideal mammalian expression hosts for recombinant protein production.The medium used for routine cell culture requires supplemental serum for normal growth.However,due to the disadvantages of serum,such as different sources and batches,difficulty in downstream separation and purification,and potential risk of mycoplasma contamination.Therefore,serum-free culture medium is required for CHO cells to produce recombinant protein drugs to avoid the above problems.In recent years,a lot of researches have been performed on serum-free medium,and remarkable progress has been made.In this review,the characteristics of CHO cells,the basic components and functions of serum-free medium,and the functions of some special additives are reviewed.

关 键 词：CHO细胞 无血清培养基 糖基化 关键成分 

分 类 号：R91[医药卫生—药学]