Molecular identifi cation and population diff erentiation of Aurelia spp.ephyrae in sea cucumber aquaculture ponds of northern China  

作　　者：Saijun PENG Qingqing LIU Lei WANG Tingting SUN Tamara SHIGANOVA Zhijun DONG 

机构地区：[1]Muping Coastal Environment Research Station,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Center for Ocean Mega-Science,Chinese Academy of Sciences,Qingdao 266071,China [4]Shirshov Institute of Oceanology Russian Academy of Sciences,Moscow 117997 Nakhimovskyi pr.,36,Russia

出　　处：《Journal of Oceanology and Limnology》2021年第3期989-996,共8页海洋湖沼学报（英文）

基　　金：Supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA23050301);the Special Exchange Program from the Chinese Academy of Sciences,the National Natural Science Foundation of China(Nos.41576152,41876138);the Instrument Developing Project of the Chinese Academy of Sciences(No.YJKYYQ20180047);the Key Research and Development Program of Yantai(No.2018ZHGY073)。

摘　　要：Aurelia spp.ephyrae have been reported to form blooms in sea cucumber aquaculture ponds in the Bohai and Yellow Seas.To identify the species,we carried out a genetic analysis of Aurelia spp.ephyrae and medusae based on mitochondrial 16 S rRNA gene.Samples offour Aurelia sp.ephyrae populations were collected in sea cucumber aquaculture ponds and samples offour Aurelia sp.medusae populations were collected in coastal waters.Using a BLASTn search,we found that both the ephyrae collected in the aquaculture ponds and medusae collected in coastal waters belong to Aurelia coerulea.Seventeen haplotypes were recovered from the 16 S rRNA gene.The overall haplotype diversity and nucleotide diversity of the 166 A.coerulea individuals were 0.686%and 0.329%,respectively,indicating high haplotype diversity and low nucleotide diversity.Moreover,the haplotype diversity of ephyrae populations were generally lower than that of medusae populations with close sampling points.The genetic differentiation between ephyrae populations collected in the sea cucumber aquaculture ponds and A.coerulea medusae collected in coastal waters was not significant,suggesting the ephyrae populations in the sea cucumber culture ponds were part of the same genetic group as the medusae populations in the coastal waters.Phylogeographic analysis of the 16 S rRNA region revealed that there was no significant correlation between the haplotypes and the geographic distribution of populations.Pairwise fixation index values showed significant genetic differentiation and limited gene flow between A.coerulea population of Weifang and other locations.

关 键 词：Aurelia coerulea MEDUSAE ephyrae 16S r RNA gene analyzes genetic differentiation genetic variability 

分 类 号：S917.4[农业科学—水产科学]