Isolation of a novel strain of Cyanobacterium sp.with good adaptation to extreme alkalinity and high polysaccharide yield  

作　　者：Zishuo CHEN Tao LI Bingjie YANG Xuejie JIN Hualian WU Jiayi WU Yandu LU Wenzhou XIANG 

机构地区：[1]CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,Guangdong Key Laboratory of Marine Materia Medica,Institution of South China Sea Ecology and Environmental Engineering,RNAM Center for Marine Microbiology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China [2]State Key Laboratory of Marine Resource Utilization in South China Sea,College of Oceanology,Hainan University,Haikou 570228,China [3]Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458,China [4]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Oceanology and Limnology》2021年第3期1131-1142,共12页海洋湖沼学报（英文）

基　　金：Supported by Key-Area Research and Development Program of Guangdong Province(No.2020B1111030004);the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(No.GML2019ZD0406);the 13th Five-Year Plan Marine Economy Innovation Development Demonstration Project(No.BHSFS004);the Project of State Key Laboratory of Marine Resource Utilization in South China Sea(No.2018004);the Guizhou Education Department Young scientific talents Promoting Program(No.KY[2016]160);the Project of Danzi(WetCode)Group(No.DZ201501)。

摘　　要：The use of high alkaline medium is a feasible way to provide carbon source and prevent biological contamination for the outdoor cultivation of alkaliphilic microalgae and cyanobacteria.A novel cyanobacterial strain was isolated from the open pond of a marine green alga(Picochlorum sp.SCSIO-45015,Sanya,Hainan)and identified as Cyanobacterium sp.SCSIO-45682.The effects of initial sodium bicarbonate(NaHCO_(3))concentrations on the growth and biochemical composition of Cyanobacterium sp.SCSIO-45682 were investigated.The results demonstrated that Cyanobacterium sp.SCSIO-45682 had good adaptation to 16.8-g/L NaHCO_(3)(the same concentration of NaHCO_(3) used in Zarrouk medium for Spirulina).Moreover,the yields of biomass,polysaccharide,chlorophyll a(chl a),and phycocyanin increased under high NaHCO_(3) concentrations.The maximum final biomass concentration of 2.5 g/L was observed at 8.4-g/L NaHCO_(3),while the highest intracellular total saccharide content of 49.2%of dry weight(DW)and exopolysaccharide(EPS)concentration of 93 mg/L were achieved at the NaHCO_(3) concentration of 16.8 g/L.The crude protein content declined under high NaHCO_(3) concentrations,which provide a possible explanation for the accumulation of polysaccharide.This study shows a good potential of alkaliphilic Cyanobacterium sp.SCSIO-45682 as a polysaccharide feedstock.

关 键 词：alkaliphilic cyanobacterium biochemical composition Cyanobacterium sp.SCSIO-45682 high sodium bicarbonate(NaHCO_(3))concentrations POLYSACCHARIDE 

分 类 号：Q949.2[生物学—植物学]