气相色谱-三重四极杆串联质谱法测定池塘养殖水中3种丁香酚类麻醉剂  

Determination of three eugenol anesthetics in culture pond water by gas chromatography-mass spectrometry

作　　者：陆添文 柯常亮[2] 刘奇[2] 黄珂[2] 李来好[2] LU Tianwen;KE Changliang;LIU Qi;HUANG Ke;LI Laihao(College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316021;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangdong Provincial Key Laboratory of Fishery Ecology and Environment,Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,P.R.China,Guangzhou 510300)

机构地区：[1]浙江海洋大学食品与药学学院,舟山316021 [2]中国水产科学研究院南海水产研究所广东省渔业生态环境重点实验室农业农村部水产品加工重点实验室,广州510300

出　　处：《分析试验室》2021年第5期518-522,共5页Chinese Journal of Analysis Laboratory

基　　金：中央级公益性科研院所基本科研业务费专项(2018ZD01);国家农产品质量安全风险评估重大专项(GJFP2019028);广东省现代农业产业技术体系创新团队建设专项(2020KJ151)资金资助。

摘　　要：建立了池塘养殖水中丁香酚、甲基丁香酚和异丁香酚等3种丁香酚类麻醉剂的残留测定方法。采用正己烷提取,苯基固相萃取柱净化,气相色谱-三重四极杆串联质谱仪进行测定。结果显示,丁香酚、甲基丁香酚和异丁香酚检测限分别为0.05,0.03,0.13μg/L,定量限分别为0.17,0.10,0.43μg/L;在1~200μg/L质量浓度范围内,3种化合物线性良好,相关系数r>0.9996;丁香酚、甲基丁香酚和异丁香酚回收率范围分别为98.5%~101.6%,98.5%~104.6%和98.4%~101.3%,相对标准偏差范围分别为2.3%~5.3%,3.2%~4.1%和2.4%~3.6%。方法能够满足养殖水中丁香酚、甲基丁香酚和异丁香酚同时测定的需要。A method was developed to simultaneously determine the residue of eugenol,methyl eugenol and isoeugenol in culture pond water.In this way,sample was extracted by n-hexane,purified by phenyl solid phase extraction column,and finally analyzed by gas chromatography coupled to tandem mass spectrometry.The results showed that the limits of detection(LOD)of eugenol,methyl eugenol and isoeugenol were 0.05,0.03,0.13μg/L,respectively.The limits of qualification(LOQ)were 0.17,0.10,0.43μg/L,respectively.Furthermore,the calibration curves displayed good linearity ranging from 1 to 200μg/L with the correlation coefficients greater than 0.9996.Spiked at 0.2,2,10μg/L,the recoveries of the target compounds were ranged from 98.5%to 101.6%for eugenol,98.5%to 104.6%for methyleugenol,98.4%to 101.3%for isoeugenol,respectively.And their relative standard deviations were from 2.3%to 5.3%,3.2%to 4.1%,and 2.4%to3.6%,respectively.The developed method was suitable for the simultaneous determination of eugenol,methyleugenol,and isoeugenol in culture pond water.

关 键 词：气相色谱三重四极杆串联质谱 固相萃取 养殖水 丁香酚 甲基丁香酚 异丁香酚 

分 类 号：S94[农业科学—水产养殖] O657.63[农业科学—水产科学]