Molecular characterization of the ryanodine receptor from Adoxophyes orana and its response to lethal and sublethal doses of chlorantraniliprole  

作　　者：SUN Li-na LIU Yan-di ZHANG Huai-jiang YAN Wen-tao YUE Qiang QIU Gui-sheng 

机构地区：[1]Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng 125100,P.R.China

出　　处：《Journal of Integrative Agriculture》2021年第6期1585-1595,共11页农业科学学报（英文版）

基　　金：the National Key R&D Program of China(2017YFD0200300);the Fundamental Research Funds for Central Non-profit Scientific Institution,China(1610182019011)。

摘　　要：The insect ryanodine receptor(RyR)is a novel target of the anthranilic and phthalic insecticides,which have high activity against lepidopteran insects.Several diamide insecticides have been used to control pests in orchards in China.To enhance our understanding of the effects of diamides on RyRs,full-length cDNAs were isolated and characterized from the summer fruit tortrix moth,Adoxophyes orana,which is the most severe pest of stone and pome trees worldwide.In addition,the modulation of AoRyRmRNA expression by diamide insecticides was investigated.The AoRyRmRNA obtained had an open reading frame(ORF)of 15402 bp nucleotides encoding 5113 amino acids,and shared high and low identity with its orthologs in other insects and mammals of 77–92 and 45–47%identity,respectively.One alternative splice site with two exclusive exons was revealed in AoRyR(a/b).The usage of exon was more frequent in eggs and larvae than in pupae and adults.Quantitative real-time reverse transcription PCR(qRT-PCR)showed that AoRyRmRNA was expressed at all developmental stages,especially in eggs,male pupae and male adults.The expression levels of AoRyRmRNA in the whole body were up-regulated markedly after 3 rd instar larvae were treated with chlorantraniliprole at LC_(10),LC_(20)and LC_(50)dosages.The results could provide the basis for further functional studies of Ao Ry R and for the development of new chemicals with selective activity against insects.

关 键 词：ryanodine receptor Adoxophyes orana CHLORANTRANILIPROLE mRNA expression 

分 类 号：TQ453[化学工程—农药化工]